Interactions and Effects on Cysteine Synthase Activity of Aminooxyacetate and Boc-Aminooxyacetate on the Bioherbicides <i>Colletotrichum truncatum</i>and <i>Alternaria cassia</i>and Their Weed Hosts

作     者：Robert E. Hoagland Kangetsu Hirase C. Douglas Boyette Robert E. Hoagland;Kangetsu Hirase;C. Douglas Boyette

作者机构：Crop Production Systems Research Unit USDA-ARS Stoneville USA Functional Chemicals Laboratory Mitsui Chemicals Chiba Japan Biological Control of Pests Research Unit USDA-ARS Stoneville USA 

出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)

年 卷 期：2021年第12卷第5期

页      面：759-770页

学科分类：07[理学] 0701[理学-数学] 070101[理学-基础数学] 

主　　题：Aminooxyacetate Bioherbicide Cysteine Synthase Pyridoxal Phosphate Antagonist Sicklepod Senna obtusifolia Hemp Sesbania Sesbania exaltata Transaminase 

摘      要：Aminooxyacetate (AOA) is a pyridoxal phosphate antagonist that inhibits various plant enzymes (including transaminases) which require pyridoxal phosphate as a cofactor and it exhibits phytotoxic and herbicidal properties. We examined AOA and its analog, N-t-butoxycarbonyl-AOA (Boc-AOA) for phytotoxicity, interactions with weed pathogens (bioherbicides), and effects on an important pyridoxal requiring enzyme, cysteine synthase (CS, E.C. 4.***.99.8). Studies were performed on two weeds, i.e., hemp sesbania [Sesbania exaltata (Raf.) Rybd. Ex A.W. Hill] and sicklepod (Senna obtusifolia), and two pathogens, (Colletotrichum truncatum and Alternaria cassiae), that are bioherbicidal agents against hemp sesbania and sicklepod, respectively. Pathogenicity tests, and assays for extractable, and in vitro CS activities were utilized. Phytotoxicity bioassays indicated that the bulky t-butoxycarbonyl moiety substitution on the AOA molecule did not substantially hinder expression of biological activity of Boc-AOA in these tests. Generally, spray application of the compounds to young dark-grown seedlings caused little growth effects, but root-feeding of the chemicals reduced growth