sLe^(x) expression in invasive micropapillary breast carcinoma is associated with poor prognosis and can be combined with MUC1/EMA as a supplementary diagnostic indicator

作     者：Yawen Song Hui Sun Kailiang Wu Jianke Lyu Jingyue Zhang Feng Gu Yongjie Ma Beibei Shen Chijuan Wang Xiaojiao Chen Jing Xu Weidong Li Fangfang Liu Li Fu Yawen Song;Hui Sun;Kailiang Wu;Jianke Lyu;Jingyue Zhang;Feng Gu;Yongjie Ma;Beibei Shen;Chijuan Wang;Xiaojiao Chen;Jing Xu;Weidong Li;Fangfang Liu;Li Fu

作者机构：Department of Breast Cancer Pathology and Research LaboratoryTianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerKey Laboratory of Breast Cancer Prevention and TherapyTianjin Medical UniversityMinistry of EducationBreast Cancer Innovation Team of the Ministry of EducationState Key Laboratory of Breast Cancer ResearchTianjin 300060China 

出 版 物：《Cancer Biology & Medicine》 (癌症生物学与医学（英文版）)

年 卷 期：2021年第18卷第2期

页      面：477-489页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by funding from the National Natural Science Foundation of China (Grant Nos. 81672637 and 81872164) 

主　　题：Invasive micropapillary carcinoma polarity reversal diagnostic indicator EMA sLe^(x) 

摘      要：Objective: Mucin 1(MUC1/EMA) and sialyl Lewis X(sLe^(x)) indicate polarity reversal in invasive micropapillary carcinoma(IMPC). The purpose of this study was to evaluate the expression of MUC1/EMA and sLe^(x) and to assess their diagnostic and prognostic value in patients with ***: The expression of sLe^(x) and MUC1/EMA in 100 patients with IMPC and a control group of 89 patients with invasive ductal carcinoma not otherwise specified(IDC-NOS) were analyzed with IHC. Fresh tumor tissues were collected from patients with IMPC or IDC-NOS for primary culture and immunofluorescence ***: The rate of nodal metastasis was higher in patients with IMPC than those with IDC-NOS, and IMPC cells tended to express more sLe^(x) and MUC1/EMA in the cytomembranes(the stroma-facing surfaces of the micropapillary clusters) than IDC-NOS cells. In IMPC, high cytomembrane expression of sLe^(x), but not MUC1/EMA, indicated poor prognosis. In addition, among the 100 patients with IMPC, 10 patients had sLe^(x)+/EMA-expression patterns, and 8 patients had sLe^(x)-/EMA+ expression patterns. The primary IMPC cells were suspended, non-adherent tumor cell clusters, whereas the primary IDC cells were adherent tumor cells. Immunofluorescence analysis showed that MUC1/EMA and sLe^(x) were co-expressed on the cytomembranes in IMPC cell clusters and in the cytoplasm in IDC-NOS ***: sLe^(x) can be used as a prognostic indicator and can be combined with MUC1/EMA as a complementary diagnostic indicator to avoid missed IMPC diagnosis.