Characterization of a Cyclophilin cDNA from Soybean Cells

作     者：阚云超 刘士旺 郭泽建 李德葆 

作者机构：浙江大学生物技术研究所杭州310029 

出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)

年 卷 期：2002年第44卷第2期

页      面：173-176页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：国家自然科学基金 ( 39980 0 33 30 0 70 493) 

主　　题：cyclophilin cDNA elicitation soybean cells 

摘      要：A cDNA clone encoded for cyclophilin (GmCyp1) was isolated by RT-PCR method from suspension-cultured soybean (Glycine max L.) cells. The deduced amino acid sequence was 91% identical to a kidney bean cyclophilin in the open reading frame of the gene. Results from Southern blotting analysis suggests that the GmCyp1 belong to a small gene family in soybean cells. The time course of GmCyp1 mRNA accumulation upon treatment of elicitor from yeast extract did not show significant change in the time period examined. The data suggest that the GmCyp1 was not regulated much by biotic factors. A possible role of the cyclophilin in the plant-pathogen interaction was discussed.