Mutational and Phylogenetic Analysis of <i>nfxB</i>Gene in Multidrug-Resistant Clinical Isolates of <i>Pseudomonas aeruginosa</i>Hyperexpressing MexCD-OprJ Efflux Pump

作     者：Manju Suresh Nithya Narayanan Kollancheri Puthurath Vimal Pullampara Rajamma Jayasree Panickassery Ramakrishnan Manish Kumar 

作者机构：Department of Biotechnology University of Calicut Malappuram Kerala India Department of Botany University of Calicut Malappuram Kerala India School of Health Sciences University of Calicut Malappuram Kerala India 

出 版 物：《Advances in Microbiology》 (微生物学（英文）)

年 卷 期：2019年第9卷第12期

页      面：993-999页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Multidrug-Resistant Pseudomonas aeruginosa Efflux Pump Regulatory Gene Mutational Variations Phylogenetic Analysis 

摘      要：The present study focused on MexCD-OprJ efflux pump and its regulatory gene nfxB in multidrug resistant (MDR) clinical isolates of Pseudomonas aeruginosa collected from Kerala, South India. Semi-quantitative reverse transcription-PCR technique was employed to detect hyperexpression of the efflux pump gene, mexD. Amplicons from nfxB gene of isolates hyperexpressing the efflux pump were sequenced for mutational and phylogenetic analysis. Among 29 isolates of MDR P. aeruginosa, increased mexD transcription was detected in 10.3% of the isolates when compared with P. aeruginosa reference strain, PAO (MTCC-3541). Various synonymous and non-synonymous mutations in nfxB regulatory gene sequences were detected. Notably, mutations detected in the strains designate Pa6 and Pa7 have been found to be novel and are hitherto unreported in GenBank data base. The genetic divergence and homogeneity of the nfxB regulatory gene sequences of mexCD-oprJ operon were clearly apparent in the phylogram generated employing similar sequences retrieved from the public database.