Vanillin and its derivatives,potential promising antifungal agents,inhibit Aspergillus flavus spores via destroying the integrity of cell membrane rather than cell wall

作     者：Qian Li Xiaoman Zhu Qian Li;Xiaoman Zhu

作者机构：Henan Key Laboratory of Cereal and Oil Food Safety Inspection and ControlCollege of Food Science and EngineeringHenan University of Technology 

出 版 物：《Grain & Oil Science and Technology》 (粮油科技（英文版）)

年 卷 期：2021年第4卷第2期

页      面：54-61页

核心收录：

学科分类：090403[农学-农药学(可授农学、理学学位）] 09[农学] 0904[农学-植物保护] 

基　　金：the financial support of the Doctor Research Fund of Henan University of Technology(2019BS019) the Natural Science Research Projects of Education Department of Henan Province(21A550005) the Innovative Funds plan of Henan University of Technology(2020ZKCJ17) 

主　　题：Aspergillus flavus Vanillin o-Vanillin HMB 2-Hydroxy-5-methoxybenzaldehyde Antifungal mechanism 

摘      要：To study the structure-antifungal activity of vanillin against Aspergillus flavus(***),the susceptibilities of *** to vanillin and its isomers(o-vanillin,2-hydroxy-4-methoxybenzaldehyde(HMB),2-hydroxy-5-methoxybenzaldehyde)and the possible antifungal mechanisms have been *** the four volatile aldehydes could inhibit the germination of spores,and the minimum inhibitory concentrations(MICs)of them were in this order:vanillin(200μg/mL),o-vanillin(100μg/mL),2-hydroxy-5-methoxybenzaldehyde(100μg/mL),HMB(70μg/mL).The minimum fungicidal concentrations(MFCs)of them were in this order:vanillin(240μg/mL),o-vanillin(160μg/mL),HMB(140μg/mL),2-hydroxy-5-methoxybenzaldehyde(140μg/mL).Spore size was arrested at 0 h with the treatment of the four volatile *** of the four volatile aldehydes on the cell wall and cell membrane integrity of A flavus were observed by calcofluor white(CW)staining and propidium iodide(PI)*** results showed that HMB exerted the strongest antifungal and fungicidal effects on the growth of *** four volatile aldehydes had little influence on cell wall integrity after 3-hour treatment,however,they could strongly damage the cell membrane *** the four volatile aldehydes could effectively prevent the growth of *** on peanut *** antifungal mechanisms of the four volatile aldehydes provide theoretical foundations for their development of new antifungal agents.