Two novel gene-specific markers at the Pik locus facilitate the application of rice blast resistant alleles in breeding

作     者：TIAN Da-gang CHEN Zi-qiang LIN Yan CHEN Zai-jie LUO Jia-mi JI Ping-sheng YANG Li-ming WANG Zong-hua WANG Feng TIAN Da-gang;CHEN Zi-qiang;LIN Yan;CHEN Zai-jie;LUO Jia-mi;JI Ping-sheng;YANG Li-ming;WANG Zong-hua;WANG Feng

作者机构：Fujian Key Laboratory of Genetic Engineering for Agriculture/Biotechnology Research InstituteFujian Academy of Agricultural SciencesFuzhou 350003P.R.China State Key Laboratory of Ecological Pest Control for Fujian and Taiwan CropsCollege of Life ScienceFujian Agriculture and Forestry UniversityFuzhou 350002P.R.China College of Biology and the EnvironmentNanjing Forestry UniversityNanjing 210037P.R.China Department of Plant PathologyUniversity of GeorgiaTiftonGA 31793USA 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第6期

页      面：1554-1562页

核心收录：

学科分类：09[农学] 090401[农学-植物病理学] 0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 0904[农学-植物保护] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：the National Natural Science Foundation of China(31640006) the China Postdocotral Science Foundation(2019M662219) the Youngth Program of Fujian Academy of Agricultural Sciences(YC2019004) the Projection of Public Welfare of Fujian Province(2017R1019-10)。 

主　　题：rice blast disease molecular marker Pik-p Pi-1 K-type alleles 

摘      要：Blast,a disease caused by Magnaporthe oryzae,is a major constraint for rice production worldwide.Introgression of durable blast resistance genes into high-yielding rice cultivars has been considered a priority to control the disease.The blast resistance Pik locus,located on chromosome 11,contains at least six important resistance genes,but these genes have not been widely employed in resistance breeding since existing markers hardly satisfy current breeding needs due to their limited scope of application.In this study,two PCR-based markers,Pikp-Del and Pi1-In,were developed to target the specific In Del(insertion/deletion)of the Pik-p and Pi-1 genes,respectively.The two markers precisely distinguished Pik-p,Pi-1,and the K-type alleles at the Pik locus,which is a necessary element for functional genes from rice varieties.Results also revealed that only several old varieties contain the two genes,of which nearly half carry the K-type alleles.Therefore,these identified varieties can serve as new gene sources for developing blast resistant rice.The two newly developed markers will be highly useful for the use of Pik-p,Pi-1 and other resistance genes at the Pik locus in markerassisted selection(MAS)breeding programs.