Towards enriching and isolation of uncultivated archaea from marine sediments using a refined combination of conventional microbial cultivation methods

作     者：Haining Hu Vengadesh Perumal Natarajan Fengping Wang 

作者机构：State Key Laboratory of Microbial MetabolismSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai200240China School of OceanographyShanghai Jiao Tong UniversityShanghai200240China 

出 版 物：《Marine Life Science & Technology》 (海洋生命科学与技术（英文）)

年 卷 期：2021年第3卷第2期

页      面：231-242页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：We would like to thank Dr.Tiantian Yu for valuable comments in modifying the paper and Dr.Mingyang Niu for help with the Archaeal 16S rRNA gene analyses.This work is supported by State Key Research and Development Project of China Grant(2016YFA0601102) China Ocean Mineral Resources R&D Association(COMRA)project(DY135-B2-12) the Natural Science Foundation of China(Grants No.41525011,91751205) the Senior User Project of RV KEXUE(KEXUE2019GZ06) 

主　　题：Microbial cultivation Marine sediments Uncultivated archaea Co-culture Bathyarchaeota 

摘      要：The archaea that can be readily cultivated in the laboratory are only a small fraction of the total diversity that exists in *** molecular ecology methods,such as metagenomic sequencing,can provide valuable information independent of cell cultivation,it is only through cultivation-based experiments that they may be fully characterized,both for their physiological and ecological ***,we report our efforts towards enriching and isolation of uncultivated archaea from marine sediments using a refined combination of conventional microbial cultivation ***,cells were retrieved from the sediment samples through a cell extraction procedure and the sediment-free mixed cells were then divided into different size-range fractions by successive filtration through 0.8µm,0.6µm and 0.2µm *** 16S rRNA gene analyses indicated noticeable retention of different archaeal groups in different *** each fraction,supplementation with a variety of defined substrates(e.g.,methane,sulfate,and lignin)and stepwise dilutions led to highly active enrichment cultures of several archaeal groups with Bathyarchaeota most prominently ***,using a roll-bottle technique,three co-cultures consisting of Bathyarchaeota(subgroup-8)and a bacterial species affiliated with either Pseudomonas or Glutamicibacter were *** results demonstrate that a combination of cell extraction,size fractionation,and roll-bottle isolation methods could be a useful protocol for the successful enrichment and isolation of numerous slow-growing archaeal groups from marine sediments.