Comparative study on production and characterisation of extracellular polymeric substances(EPS)using activated sludge fortified with crude glycerol from different biodiesel companies
作者机构：INRS EauTerre et Environnement490rue de la CouronneQuebec CityQC G1K 9A9Canada BOSK BioproductsQuebec CityQCCanada
出 版 物：《Systems Microbiology and Biomanufacturing》 (系统微生物学与生物制造（英文）)
年 卷 期：2021年第1卷第2期
页 面：208-222页
学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学]
基 金：BIO-LIQ Natural Sciences and Engineering Research Council of Canada, NSERC, (A4984) Canada Research Chairs
主 题：Exopolysaccharides Bacterial polymers Slime Crude glycerol Activated sludge Impurities
摘 要：Extracellular polymeric substances(EPS)are eco-friendly and economical biopolymers with a significant role in pollutant removal,bio-flocculation,settling and dewatering of activated *** this study,growth profile and EPS production by an EPS-producing microbial strain(isolated from wastewater sludge or WWS)was studied using activated sludge fortified with *** study compared three crude glycerol samples as carbon sources from different biodiesel companies(BIO-LIQ,BIOCARDEL,ROTHSAY)and their effect on EPS production and characterisation was *** maximum slime EPS(S-EPS)concentration(12.34 g/L)was produced when sludge fortified with BIO-LIQ crude glycerol was used,higher than pure glycerol(10 g/L)at 72 *** S-EPS was enhanced(16 g/L)when purified BIO-LIQ glycerol(by acid treatment)was *** was also observed that S-EPS concentration increased 1.6-2.6 times by using sludge fortified with glycerol as compared to only glycerol(no sludge)as EPS production *** addition,the S-EPS produced from different glycerol samples yielded different characteristics in terms of protein-carbohydrate content,flocculation activity and *** S-EPS from purified BIO-LIQ glycerol(with sludge)gave maximum flocculation activity and dewaterability i.e.93.71%and 60.2%*** comparison between Ca(OH)2 and NaOH used as alkaline sludge pre-treatment methods revealed that the EPS produced from Ca(OH)_(2)-treated sludge fortified with glycerol solutions to be better in terms of protein content,flocculation activity and *** composition analysis using Fourier Transform Infrared Spectroscopy(FT-IR)revealed the presence of distinct functional groups(carboxyl,hydroxyl,amine groups)in the produced S-EPS.
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