Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin

作     者：Jinxiu Wang Suze Ma Wei Ding Tuo Chen Qi Zhang Jinxiu Wang;Suze Ma;Wei Ding;Tuo Chen;Qi Zhang

作者机构：State Key Laboratory of Cryospheric ScienceKey Laboratory of Extreme Environmental Microbial Resources and EngineeringNorthwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhouGansu730000 China Department of ChemistryFudan UniversityShanghai200433 China University of Chinese Academy of SciencesBeijing100049 China State Key Laboratory of Microbial MetabolismSchool of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai200240 China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2021年第39卷第7期

页      面：1923-1926页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：This work is supported in part by grants from the National Key Research and Development Program(Nos.2018Y F A0900402 and 2016Y F A0501302) from the National Natural Science Foundation of China(Nos.21822703 and 21921003) from the MOST Inter-Government International Science and Technology Innovation Cooperation project(No.2019YFE0121100) 

主　　题：Biosynthesis Flavin Flavoprotein Oxidase Enzyme mechanism 

摘      要：Main observation and conclusion The aminoglycoside antibiotic apramycin contains a unique bicyclic octose moiety,and biosynthesis of this moiety involves an oxidoreductase *** other known“Qseries proteins involved in aminoglycosides biosynthesis,AprQ does not work with an aminotransferase partner,and performs a four-electron oxidation that converts a CH2OH moiety to a carboxylate group.