First Isolation and Confirmation of Sterol Based on β-sitosterol Skeleton from the Leaves of Podocarpusnagi Planted in Fujian, Preliminary in vitro Anticancer Activity and the Crystal Structure

作     者：YANG Yang YONG Jian-Ping OLAGOKE Zacchaeus Olatunde LU Can-Zhong 杨洋;雍建平;奥拉特德·奥拉戈克·扎克斯;卢灿忠

作者机构：Fujian Institute of Research on the Structure of MatterHaixi InstituteChinese Academy of SciencesFuzhou 350002China Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China Xiamen Institute of Rare-earth MaterialsHaixi InstituteChinese Academy of SciencesXiamen 361021China Shanghai Tech UniversityShanghai 201210China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Chinese Journal of Structural Chemistry》 (结构化学（英文）)

年 卷 期：2021年第40卷第5期

页      面：653-658,531页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：financially supported by the National Natural Science Foundation of China(21875252) Self-created Area Project of Major Science Technology Innovation Platform of Xiamen(3502ZCQ20171002) 

主　　题：Podocarpusnagi crystal structure sterols isolation anticancer evaluation 

摘      要：The crystal of(24 R)-3β,5α-dihydroxy-24-ethyl-5α-cholestan-6-one(1) was isolated from the leaves of *** planted in Fujian for the first time with the same skeleton as 26,27-dinorcholest-5-en-3-β-ol(2) andβ-sitosterol(3) that have been reported *** 1 crystallizes in monoclinic,space group P2_(1) with a=10.8482(4),b=7.3671(3),c=33.7860(15) A,β=93.103(4)°,V=2696.21(19) A^(3),Z=75,M_(r)=446.70,ρ_(calc)=1.145 g/cm^(3),F(000)=1032,μ=0.572 mm^(-1),GOOF=1.034,the final R=0.0467 and wR=0.1129 for 6989 observed reflections with I 2σ(Ⅰ).Compound 2 was selected to evaluate for their preliminary in vitro anticancer activity against four cancer cell lines for the first *** results showed that compound 2 exhibited great inhibition against gastric cancer,breast cancer MCF-7,lung cancer A549 and Helacell lines with the inhibitions of89.16% ± 1.17,97.02%±0.53,47.20%±2.58 and 36.89%±1.22 respectively at the concentration of 1.4×10^(-2) M,which means that we found the new anticancer compound in this plant medicine.