Plant-based,adjuvant-free,potent multivalent vaccines for avian influenza virus via Lactococcus surface display

作     者：Shi-Jian Song Gyeong-Im Shin Jinyong Noh Jiho Lee Deok-Hwan Kim Gyeongryul Ryu Gyeongik Ahn Hyungmin Jeon Hai-Ping Diao Youngmin Park Min Gab Kim Woe-Yeon Kim Young-Jin Kim Eun-Ju Sohn Chang Seon Song Inhwan Hwang Shi-Jian Song;Gyeong-Im Shin;Jinyong Noh;Jiho Lee;Deok-Hwan Kim;Gyeongryul Ryu;Gyeongik Ahn;Hyungmin Jeon;Hai-Ping Diao;Youngmin Park;Min Gab Kim;Woe-Yeon Kim;Young-Jin Kim;Eun-Ju Sohn;Chang Seon Song;Inhwan Hwang

作者机构：Department of Life SciencePohang University of Science and TechnologyPohang37673 Korea College of Pharmacy and Research Institute of Pharmaceutical ScienceGyeongsang National UniversityJinju660-701 Korea KCAV Inc.Gwangjin-gu05029 Korea Avian Disease LaboratoryCollege of Veterinary MedicineKonkuk UniversitySeoul05029 Korea Division of Applied Life Science(BK21 PLUS)Institute of Agriculture&Life SciencesGyeongsang National UniversityJinju52828 Korea BioappInc.Pohang Technopark ComplexPohang37668 Korea 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第8期

页      面：1505-1520页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：This work was supported by the Ministry of Trade,Industry and Energy(grant number,10063301) by the National Research Foundation grants funded by the Ministry of Science and Information Technology(No.2019R1A2B5B-03099982 and 2019R1A2C1087207),Korea 

主　　题：bacteria-like particle(BLP) H5N6 H9N2 Lactococcus lactis multi-valent vaccine plant-based vaccine trimeric HA 

摘      要：Influenza epidemics frequently and unpredictably break out all over the world,and seriously affect the breeding industry and human *** and live attenuated viruses have been used as protective vaccines but exhibit high risks for *** vaccines enjoy high biosafety and specificity but have a few weak points compared to inactivated virus or live attenuated virus vaccines,especially in low *** this study,we developed a new subunit vaccine platform for a potent,adjuvant-free,and multivalent *** ectodomains of hemagglutinins(HAs)of influenza viruses were expressed in plants as trimers(tHAs)to mimic their native *** in plant extracts were directly used without purification for binding to inactivated Lactococcus(iLact)to produce iLact-tHAs,an antigen-carrying bacteria-like particle(BLP).tHAs BLP showed strong immune responses in mice and chickens without ***,simultaneous injection of two different antigens by two different formulas,t^(HAH5N6+H9N2) BLP or a combination of t^(HAH5N6) BLP and t^(HAH9N2) BLP,led to strong immune responses to both *** on these results,we propose combinations of plant-based antigen production and BLP-based delivery as a highly potent and cost-effective platform for multivalent vaccination for subunit vaccines.