Photooxidation crosslinking to recover residual stress in decellularized blood vessel

作     者：Jintao Wang Lingwen Kong Alidha Gafur Xiaobo Peng Natalia Kristi Jing Xu Xingshuang Ma Nan Wang Rose Humphry Colm Durkan Haijun Zhang Zhiyi Ye Guixue Wang 

作者机构：Key Laboratory of Biorheological Science and Technology(Chongqing University)Ministry of EducationState and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China Department of Cardiothoracic SurgeryCentral Hospital of Chongqing UniversityChongqing Emergency Medical CenterChongqing 400014China The Nanoscience CentreUniversity of CambridgeCambridge CB30FFUK National Local Joint Engineering Laboratory for Biomedical Material ModificationDezhouShandong 251100China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2021年第8卷第2期

页      面：13-25页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：This work was supported by grants from the National Key R&D Program(2016YFC1101101,2016YFC1102305) Preface Technology Project of Chongqing(cstc2018jcyjAX0530,cstc2019jcyj-msxmX0329) Fundamental Research Funds for Central Universities(2018CDXYSW0023) the National Natural Science Foundation of China(30570453,31971242) We are also thankful for the support from the Chongqing Engineering Laboratory in Vascular Implants,the Public Experiment Center of State Bioindustrial Base(Chongqing),China 

主　　题：residual stress opening angle mechanical property decellularized artery photooxidation crosslinking finite element method 

摘      要：Decellularization method based on trypsin-digestion is widely used to construct small diameter vascular ***,this method will reduce the opening angle of the blood vessel and result in the reduction of residual *** stress reduced has an adverse effect on the compliance and permeability of small diameter vascular *** improve the situation,acellular blood vessels were treated with glutaraldehyde and photooxidation crosslinking respectively,and the changes of opening angle,circumferential residual strain of native blood vessels,decellularized arteries and crosslinked blood vessels were measured by means of histological examination,scanning electron microscopy(SEM)and transmission electron microscopy(TEM)in this *** opening angle of decellularized arteries significantly restored after photooxidation crosslinking(P紏0.0216),while that of glutaraldehyde crosslinking blood vessels *** elastic fibers inside the blood vessels became densely rearranged after photooxidation *** results of finite element simulation showed that the residual stress increased with the increase of opening *** this study,we found at the first time that photooxidation crosslinking method could significantly increase the residual stress of decellularized vessels,which provides biomechanical support for the development of new biomaterials of vascular grafts.