In <i>Vitro</i>Clonal Propagation from Adult Material of a Savannah Species of Socio-Economic Importance: <i>Annona senegalensis</i>Pers.

作     者：Oumar Ba Abraham Diémé Mame Ourèye Sy Oumar Ba;Abraham Diémé;Mame Ourèye Sy

作者机构：Laboratoire Campus de Biotechnologies Végétales Département de Biologie Végétale Faculté des Sciences & Techniques Université Cheikh Anta Diop Dakar Sénégal 

出 版 物：《Agricultural Sciences》 (农业科学（英文）)

年 卷 期：2021年第12卷第4期

页      面：370-386页

学科分类：07[理学] 0701[理学-数学] 070101[理学-基础数学] 

主　　题：Annona senegalensis Adult Material Micropropagation 6-Benzylaminopurine 1-Naphthaleneacetic Acid Indole-3-Butyric Acid 

摘      要：Annona senegalensis (Pers.) or Annone from Senegal is a species found in the West African savannah and para-littoral sands. It offers real possibilities of socio-economic use, particularly in the medical, nutritional, ecosystem conservation and poverty alleviation fields. However, this wild species remained not fully exploited despite its potentialities. As a result, there is a need to regenerate this species;therefore, the in vitro propagation from adult material was undertaken. For this purpose, axillary nodes from plant regrowth taken from elderly subjects were aseptically introduced into different media enriched or not with phytohormones. Among the 3 culture media tested, that of Murashige and Skoog (MS, 1962) was the most reactive and made it possible to develop a micropropagation protocol for adult material of A. senegalensis. Thus, the introduction of these explants in media containing cytokinins (BAP and Kinetin) significantly increased the reactivity compared to media without hormones. If the best average numbers of shoots (2.3) and nodes (5.3) are obtained in MS + BAP 2 mg·L−1, with a reactivity of 91.66%, the addition of 0.1 mg·L−1 NAA gave the best average length (8.25 cm) of vitroplants. An induction time of 3 days into darkness with IBA at 50 mg·Lspan style=white-spac