Caffeic acid and protocatechuic acid modulate Nrf2 and inhibit Ehrlich ascites carcinomas in mice
作者机构:Center of Excellence in Molecular Biology and Regenerative MedicineDepartment of BiochemistryJSS Medical CollegeJSS Academy of Higher Education&ResearchMysore-570015KarnatakaIndia SRM Research InstituteDepartment of BiotechnologySchool of BioengineeringSRM Institute of Science and TechnologyKattankulathur-603203Tamil NaduIndia
出 版 物:《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志(英文版))
年 卷 期:2021年第11卷第6期
页 面:244-253页
核心收录:
学科分类:0710[理学-生物学] 0831[工学-生物医学工程(可授工学、理学、医学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金:Indian Council of Medical Research ICMR (:3/2/2/105/2019/NCD-III :3/2/2/5/2018/online Onco Fship/NCD-III)
主 题:Ehrlich ascites carcinomas Nrf2 Protocatechuic acid Caffeic acid NQO1 activity
摘 要:Objective:To assess the nuclear factor-erythroid 2-related factor-2(Nrf2)modulatory effect of caffeic acid and protocatechuic acid and determine the anti-tumor activity of these phenolic compounds against Ehrlich ascites carcinoma growth in mice.Methods:Antioxidant activity of protocatechuic acid and caffeic acid was assessed using ferric reducing antioxidant power(FRAP)and 2,2-diphenyl-1-picrylhydrazyl(DPPH).Nrf2 activation potential of phenolic compounds was tested by quantitative realtime polymerase chain reaction,and luciferase complementation reporter assays.In vivo efficacy was tested using the Ehrlich ascites carcinoma model.Results:FRAP and DPPH radical scavenging assays showed that caffeic acid and protocatechuic acid were more potent compared with cinnamic acid and benzoic acid.Luciferase complementation reporter assays identified caffeic acid and protocatechuic acid as the activators of Nrf2.Both caffeic acid and protocatechuic acid upregulated the expression of Nrf2 target genes heme oxygenase-1(HO-1),glutamate-cysteine ligase catalytic subunit(GCLC),and glutamate-cysteine ligase modifier subunit(GCLM)and the activity of NAD(P)H:quinone oxidoreductase 1(NQO1)when tested on HCT-116 cells using a cell-based assay system at 9 h.In addition,intraperitoneal administration of caffeic acid and protocatechuic acid to Ehrlich ascites carcinoma bearing mice suppressed tumor growth and angiogenesis.Conclusions:Caffeic acid and protocatechuic acid can modulate Nrf2 and inhibit Ehrlich ascites carcinoma cells.