Caffeic acid and protocatechuic acid modulate Nrf2 and inhibit Ehrlich ascites carcinomas in mice

作     者：Venugopal R.Bovilla Preethi G.Anantharaju Sireesh Dornadula Prashanthkumar M.Veeresh Mahadevaswamy G.Kuruburu Vidya G.Bettada Kunka Mohanram Ramkumar SubbaRao V.Madhunapantula Venugopal R.Bovilla;Preethi G.Anantharaju;Sireesh Dornadula;Prashanthkumar M.Veeresh;Mahadevaswamy G.Kuruburu;Vidya G.Bettada;Kunka Mohanram Ramkumar;SubbaRao V.Madhunapantula

作者机构：Center of Excellence in Molecular Biology and Regenerative MedicineDepartment of BiochemistryJSS Medical CollegeJSS Academy of Higher Education&ResearchMysore-570015KarnatakaIndia SRM Research InstituteDepartment of BiotechnologySchool of BioengineeringSRM Institute of Science and TechnologyKattankulathur-603203Tamil NaduIndia 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2021年第11卷第6期

页      面：244-253页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Indian Council of Medical Research  ICMR  (:3/2/2/105/2019/NCD-III  :3/2/2/5/2018/online Onco Fship/NCD-III) 

主　　题：Ehrlich ascites carcinomas Nrf2 Protocatechuic acid Caffeic acid NQO1 activity 

摘      要：Objective:To assess the nuclear factor-erythroid 2-related factor-2(Nrf2)modulatory effect of caffeic acid and protocatechuic acid and determine the anti-tumor activity of these phenolic compounds against Ehrlich ascites carcinoma growth in ***:Antioxidant activity of protocatechuic acid and caffeic acid was assessed using ferric reducing antioxidant power(FRAP)and 2,2-diphenyl-1-picrylhydrazyl(DPPH).Nrf2 activation potential of phenolic compounds was tested by quantitative realtime polymerase chain reaction,and luciferase complementation reporter *** vivo efficacy was tested using the Ehrlich ascites carcinoma ***:FRAP and DPPH radical scavenging assays showed that caffeic acid and protocatechuic acid were more potent compared with cinnamic acid and benzoic *** complementation reporter assays identified caffeic acid and protocatechuic acid as the activators of *** caffeic acid and protocatechuic acid upregulated the expression of Nrf2 target genes heme oxygenase-1(HO-1),glutamate-cysteine ligase catalytic subunit(GCLC),and glutamate-cysteine ligase modifier subunit(GCLM)and the activity of NAD(P)H:quinone oxidoreductase 1(NQO1)when tested on HCT-116 cells using a cell-based assay system at 9 *** addition,intraperitoneal administration of caffeic acid and protocatechuic acid to Ehrlich ascites carcinoma bearing mice suppressed tumor growth and ***:Caffeic acid and protocatechuic acid can modulate Nrf2 and inhibit Ehrlich ascites carcinoma cells.