Identification and characterization of a monoclonal antibody blocking the SARS-CoV-2 spike protein–ACE2 interaction

作     者：MengYa Yuan YaoYing Wan Cui Liu Yue Li\ZhaoYuan Liu ChangDong Lin JianFeng Chen 

作者机构：State Key Laboratory of Cell BiologyCenter for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghaiChina School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhouChina 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2021年第18卷第6期

页      面：1562-1564页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：supported by the National Key Research and Development Program of China(2020YFA0509000 to J.F.C.) the National Natural Science Foundation of China(32030024,31830112,and 31525016 to J.F.C.and 31970702 to C.D.L.) the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA12010101 to J.F.C.) the Program of Shanghai Academic Research Leaders(19XDI 404200) National Ten Thousand Talents,the Youth Innovation Promotion Association of the Chinese Academy of Sciences(2020266) and the Young Elite Scientist Sponsorship Program by CAST(2019QNRC001) 

主　　题：ACE2 monoclonal infection 

摘      要：Because COVID-19 has spread as a global pandemic,the development of therapeutics for this disease,such as neutralizing antibodies that can efficiently block SARS-CoV-2 infection,is urgently *** the infection of host cells by SARS-CoV-2,the spike(S)glycoprotein of SARS-CoV-2 plays the most crucial roles in viral entry and cell *** S protein includes two components,the S1 and S2 *** S1 subunit is vital in determining tissue tropism and host ranges and consists of the N-terminal domain and C-terminal receptor-binding domain(RBD).1,2 Of note,the RBD of the S protein(S-RBD)supports the binding of the S protein to angiotensin-converting enzyme 2(ACE2)on host cells,contributing to cell entry by SARS-CoV-2.3 Therefore,the S-RBD is widely used for the development of neutralizing antibodies,small-molecule inhibitors and vaccines.