A dual-function chemical probe for detecting erasers of lysine lipoylation

作     者：Yusheng Xie Jie Zhang Liu Yang Qingxin Chen Quan Hao Liang Zhang Hongyan Sun Yusheng Xie;Jie Zhang;Liu Yang;Qingxin Chen;Quan Hao;Liang Zhang;Hongyan Sun

作者机构：Department of ChemistryCity University of Hong KongHong KongChina Key Laboratory of Biochip TechnologyBiotech and Health CentreCity University of Hong Kong Shenzhen Research InstituteShenzhen 518057China School of Biomedical SciencesUniversity of Hong KongHong KongChina Department of Biomedical ScienceCollege of Veterinary Medicine and Life SciencesCity University of Hong KongHong KongChina 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2022年第16卷第1期

页      面：121-127页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：supported by the Science Technology and Innovation Committee of Shenzhen Municipality(Grant Nos.JCYJ20180507181654823 and JCYJ20170413141047772) the National Natural Science Foundation of China(Grant No.21778044) 

主　　题：dual-function fluorescent probe labeling photo-cross-linker lipoylation modification eraser sirtuin 

摘      要：Lysine lipoylation plays vital roles in cell metabolism and redox *** example,removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid *** the important functions of lysine lipoylation,the mechanisms for the addition and removal of this modification remain largely *** few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation *** example,immunoaffinity purification-mass spectrometry is one of such tools,which highly relies on antibody efficiency and purification ***-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation *** labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting ***,we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation(eraser)activity and interaction of lysine lipoylation with the eraser at the same *** show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant *** envision that the probe will help delineate the roles of delipoylation enzyme in biology.