Observation and analysis of morphology abnormalities in development of Oryzias melastigma embryos

作     者：Jingjing SONG Haiyi WU Kaikai LIU Wendan CHI Shanshan GE Zhipeng ZHANG Mingqi WANG Yi YU Daode YU Jingjing SONG;Haiyi WU;Kaikai LIU;Wendan CHI;Shanshan GE;Zhipeng ZHANG;Mingqi WANG;Yi YU;Daode YU

作者机构：Healthy Mariculture Engineering Research Center of Shandong ProvinceMarine Science Research Institute of Shandong ProvinceQingdao 266104China Tianjin Research Institute for Water Transport EngineeringMinistry of TransportTianjin 300456China Ocean University of ChinaQingdao 266100China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2021年第39卷第4期

页      面：1485-1499页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Supported by the National Key R&D Program of China(No.2018YFC1406406) the Science and Technology Program of Yantai(Nos.2018SFBF084,2019ZDCX018) 

主　　题：marine fish model abnormal embryogenesis blastomeres separation Kupffer’s vesicle conjoined twins 

摘      要：Fish embryos are widely used as models in toxicology,drug development,and human disease research because of their high sensitivity,observability,and operability,providing the basis for an in-depth understanding of the *** studies have indicated that birth defects are *** this study,we used Oryzias melastigma as a model to conduct a study of 185-day embryogenesis and observed self-induced non-pathological abnormal *** experienced pre-puberty stage,adolescence stage,and senescence stage,and individuals produced up to 102 eggs per ***,the fecundity was markedly reduced during the senescent *** the active egg and blastodisc stages,pseudo-fertilization and pseudo-blastocysts were *** cleavage at the 4-to 32-cell stages,we observed blastomeres separation or *** separated blastomeres formed double blastoderms,eventually resulting in conjoined *** the blastula stage,we observed abnormally increased cell volume,narrowed and elongated blastocysts,and abnormally coated *** the organogenesis stage,we observed abnormal numbers of Kupff er’s vesicles and conjoined *** in the location and number of oil droplets were observed in various development *** development was more commonly observed in fertilized eggs produced by broodstock in pre-puberty or senescence stages,which is probably related to the age of fish and the egg *** study can provide the materials for comparative analysis in toxicological and molecular studies of ***,and may provide evidence for other economic fish that produce sticky eggs.