Cytopathic effects and local immune responses in repeated neoadjuvant HSV-tk+ ganciclovir gene therapy for prostate cancer

作     者：Nobuyuki Yanagisawa Takefumi Satoh Ken-ichi Tabata Hideyasu Tsumura Yasutomo Nasu Masami Watanabe Timothy C.Thompson Isao Okayasu Yoshiki Murakumo Shiro Baba Masatsugu Iwamura 

作者机构：Department of PathologySt.Marianna University School of Medicine Yokohama-City Seibu HospitalYokohamaKanagawaJapan Department of PathologyKitasato University School of MedicineSagamiharaKanagawaJapan Department of UrologyKitasato University School of MedicineSagamiharaKanagawaJapan Takefumi Satoh Prostate ClinicMachidaTokyoJapan Department of UrologyOkayama UniversityOkayamaJapan Center for Innovative Clinical MedicineOkayama University HospitalOkayamaJapan Department of Genitourinary Medical Oncology-ResearchDivision of Cancer MedicineThe University of Texas MD Anderson Cancer CenterHoustonTXUSA Division of NutritionSchool of Health CareKiryu UniversityMidori-City GunmaJapan 

出 版 物：《Asian Journal of Urology》 (亚洲泌尿外科杂志（英文）)

年 卷 期：2021年第8卷第3期

页      面：280-288页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by Grants-in-Aid for Scientific Research(JSPS KAKENHI)grant(number 21592060) 

主　　题：Suicide genetherapy Prostatecarcinoma HSV-tk Ganciclovir Immuno histochemistry 

摘      要：Objective:Cytopathic effects and local immune response were analyzed histologically in prostatic cancer(PCa)with in situ herpes simplex virus-thymidine kinase(HSV-tk)/ganciclovir(GCV)gene therapy(GT).Methods:Four high-risk PCa patients who received HSV-tk/GCV GT were *** two cycles of intraprostatic injection of HSV-tk and administration of GCV,radical prostatectomy was ***-fixed,paraffin-embedded sections were evaluated using *** with hormone therapy(HT,n=3)or without neoadjuvant therapy(NT,n=4)that were equivalent in terms of risk were also examined as ***-positive cells were counted in at least three areas in cancer *** indices(LI)were calculated as percentage ***:ssDNA LI in GT increased,indicating apoptosis,as well as tumor-infiltrating lymphocytes and CD68-positive macrophages,compared with their *** cases showed significantly higher numbers of single-stranded DNA(ssDNA)LI,CD4/CD8-positive T cells and CD68-positive macrophages including M1/M2 macrophages than HT or NT ***,there was no significant difference in CD20-positive B cells among the types of *** were strong correlations between CD8+T cells and CD68+macrophages(ρ=0.656,p0.0001)as well as CD4+T cells and CD20+B cells(ρ=0.644,p0.0001)in PCa with ***:Enhanced cytopathic effect and local immune response might be indicated in PCa patients with HSV-tk/GCV gene therapy.