Genetic diversity and structure of the endemic and endangered species Aristolochia delavayi growing along the Jinsha River

作     者：Yu-Long Yu Hui-Chun Wang Zhi-Xiang Yu Johann Schinnerl Rong Tang Yu-Peng Geng Gao Chen Yu-Long Yu;Hui-Chun Wang;Zhi-Xiang Yu;Johann Schinnerl;Rong Tang;Yu-Peng Geng;Gao Chen

作者机构：School of Ecology and Environmental ScienceYunnan UniversityKunming650091China Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small PopulationsKunming Institute of BotanyChinese Academy of SciencesKunming650201China University of Chinese Academy of SciencesBeijing100049China Sichuan Panzhihua Cycas National Nature Reserver BureauPanzhihua617000China Department of Botany and Biodiversity ResearchUniversity of ViennaRennweg 14A-1030ViennaAustria CAS Key Laboratory for Plant Biodiversity and Biogeography of East AsiaKunming Institute of BotanyChinese Academy of SciencesKunming650201China 

出 版 物：《Plant Diversity》 (植物多样性（英文版）)

年 卷 期：2021年第43卷第3期

页      面：225-233页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0902[农学-园艺学] 090102[农学-作物遗传育种] 0713[理学-生态学] 

基　　金：Support for this study was provided by grants from the NSFC(National Natural Science Foundation of China)-Yunnan Joint fund to support key projects(No.U1602264) Yunnan Ten Thousand Talents Plan Young&Elite Talent Project to G.Chen(YNWR-QNBJ-2018-017) NSFC(National Natural Science Foundation of China)(No.31660057) Y.P.Geng Science&Technology Basic Resources Investigation Program of China for Survey and Germplasm Conservation of PSESP in Southwest China(2017FY100100)to W.B.Sun 

主　　题：Aristolochia delavayi Conservation biology Genetic diversity Genetic structure Microsatellites Genome skimming 

摘      要：The traditional medicinal plant,and endangered species Aristolochia delavayi(Aristolochiaceae)is an endemic species in China and occurs in the warm and dry areas along the Jinsha *** is also a specific host of the larvae of Byasa daemonius,a vulnerable *** this study,15 pairs of polymorphic microsatellite primers of *** were designed and screened based on the Simple Sequence Repeats(SSR)loci found by using the results of genome *** on these 15 SSR markers,the genetic diversity and structure of 193 individuals from ten natural populations were analyzed in *** comparison to other endemic and endangered plants in the region,the population of *** possess a relatively high genetic diversity(He=0.550,I=1.112).AMOVA analysis showed that 68.4%of the total genetic diversity was within populations and 31.6%of the variation occurred among *** was a significant genetic differentiation among natural populations of *** detectable,with low gene flow(Nm=0.591).This might be attributed to geographical barriers and limited seed *** test the isolation by distance(IBD),we performed Mantel test,which showed a significant correlation between the geographic and genetic *** order to cope with the possible biases caused by IBD,we additionally performed Bayesian genetic cluster analyses and principal coordinate analysis(PCoA).The final cluster analysis revealed three groups with distinct geographical *** fragmentation and limited gene flow between these populations may be the main reasons for the current genetic *** conservation of this species,we suggest to divide its populations into three protection management units,with subsequent focus on the Yongsheng and Luquan populations which experienced a genetic bottleneck event in the past.