Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota

作     者：Hua Zhou Bing Yu Jing Sun Zuohua Liu Hong Chen Liangpeng Ge Daiwen Chen Hua Zhou;Bing Yu;Jing Sun;Zuohua Liu;Hong Chen;Liangpeng Ge;Daiwen Chen

作者机构：Key Laboratory of Animal Disease-Resistance NutritionChengdu 611130SichuanChina Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Pig Industry SciencesRongchang 402460ChongqingChina Chongqing Academy of Animal SciencesRongchang 402460ChongqingChina College of Food ScienceSichuan Agricultural UniversityYa’an 625014SichuanChina 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2021年第12卷第4期

页      面：1414-1427页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：study was supported by National Natural Science Foundation of China(31730091) the National Key Research and Development Program of China(2017YFD0500503) 

主　　题：Germ-free Glucose metabolism Lipid metabolism Pig model Short-chain fatty acids 

摘      要：Background:Previous studies have shown that exogenous short-chain fatty acids(SCFAs)introduction attenuated the body fat deposition in conventional mice and ***,limited studies have evaluated the effects of exogenously introduced SCFAs on the lipid and glucose metabolism independently of the gut *** study was to investigate the effects of exogenous introduction of SCFAs on the lipid and glucose metabolism in a germ-free(GF)pig ***:Twelve hysterectomy-derived newborn pigs were reared in six sterile *** pigs were hand-fed with sterile milk powder for 21 d,then the sterile feed was introduced to pigs for another 21 *** the second 21-d period,six pigs were orally administrated with 25 mL/kg sterile saline per day and considered as the GF group,while the other six pigs were orally administrated with 25 mL/kg SCFAs mixture(acetic,propionic,and butyric acids,45,15,and 11 mmol/L,respectively)per day and regarded as FA ***:Orally administrated with SCFAs tended to increase the adiponectin concentration in serum,enhance the CPT-1 activity in longissimus dorsi,and upregulate the ANGPTL4 mRNA expression level in colon(P0.10).Meanwhile,the mRNA abundances of ACC,FAS,and SREBP-1C in liver and CD36 in longissimus dorsi of the FA group were decreased(P0.05)compared with those in the GF ***,the mRNA expression of PGC-1αin liver and LPL in longissimus dorsi tended to(P0.10)upregulate and downregulate respectively in the FA ***,oral administration of SCFAs tended to increase the protein level of GPR43(P0.10)and decrease the protein level of ACC(P0.10)in ***,oral administration of SCFAs upregulated the p-AMPK/AMPK ratio and the mRNA expressions of GLUT-2 and GYS2 in liver(P0.05).In addition,the metabolic pathway associated with the biosynthesis of unsaturated fatty acids was most significantly promoted(P0.05)by oral administration of ***:Exogenous introduction of SCFAs might attenuate t