Arabidopsis U-box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor-like protein kinases LRR1 and KIN7

作     者：Xuexue Chen Tingting Wang Amin Ur Rehman Yu Wang Junsheng Qi Zhen Li Chunpeng Song Baoshan Wang Shuhua Yang Zhizhong Gong Xuexue Chen;Tingting Wang;Amin Ur Rehman;Yu Wang;Junsheng Qi;Zhen Li;Chunpeng Song;Baoshan Wang;Shuhua Yang;Zhizhong Gong

作者机构：State Key Laboratory of Plant Physiology and BiochemistryCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop Stress BiologyInstitute of Plant Stress BiologyHenan UniversityKaifeng 475001China Key Lab of Plant Stress ResearchCollege of Life ScienceShandong Normal UniversityJi'nan 250000China School of Life SciencesInstitute of Life Science and Green DevelopmentHebei UniversityBaoding 100193China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第3期

页      面：494-509页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：supported by grants from the National Science Foundation of China(31730007) the National Major Project for Transgenic Organism Breeding,Ministry of Agriculture and Rural Affairs of the People’s Republic of China(2016ZX08009002) the National Science Foundation of China(31921001) the Beijing Outstanding University Discipline Program。 

主　　题：abscisic acid drought response E3 ligase receptor-like kinase ubiquitination 

摘      要：Both plant receptor-like protein kinases(RLKs)and ubiquitin-mediated proteolysis play crucial roles in plant responses to drought stress.However,the mechanism by which E3 ubiquitin ligases modulate RLKs is poorly understood.In this study,we showed that Arabidopsis PLANT U-BOX PROTEIN 11(PUB11),an E3 ubiquitin ligase,negatively regulates abscisic acid(ABA)-mediated drought responses.PUB11 interacts with and ubiquitinates two receptor-like protein kinases,LEUCINE RICH REPEAT PROTEIN 1(LRR1)and KINASE 7(KIN7),and mediates their degradation during plant responses to drought stress in vitro and in vivo.pub11 mutants were more tolerant,whereas Irr1 and kin7 mutants were more sensitive,to drought stress than the wild type.Genetic analyses show that the pub11 Irr1 kin7 triple mutant exhibited similar drought sensitivity as the Irr1 kin7 double mutant,placing PUB11 upstream of the two RLKs.Abscisic acid and drought treatment promoted the accumulation of PUB11,which likely accelerates LRR1 and KIN7 degradation.Together,our results reveal that PUB11 negatively regulates plant responses to drought stress by destabilizing the LRR1 and KIN7 RLKs.