Analyses of microRNAs and transcriptomes in the midgut of Spodoptera litura feeding on Brassica juncea
作者机构：Zheng School of Life SciencesSouth China Normal UniversityGuangzhouChina
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2021年第28卷第2期
页 面：533-547页
核心收录：
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：This study was supported by the Chinese National Natural Science Foundation(Grant No.31672362) the Scientific Research Cultivation Fund for Young Faculty of South China Normal University(Grant No.2014bsxm 10).
主 题：Brassica juncea midgut miRNAs RNA-Seq Spodoptera litura
摘 要：Spodoptera litura is a destructive agricultural pest in tropical and subtropical areas.Understanding the molecular mechanisms of S.litura adaptation to its preferred host plants may help identify target genes useful for pest control.We used high-throughput sequencing to characterize the expression patterns of messenger RNAs(mRNAs)and microRNAs(miRNAs)in the midgut of S.litura fed on Brassica juncea for 6 h and 48 h.A total of 108 known and 134 novel miRNAs were identified,29 miRNAs and 237 mRNAs were differentially expressed at 6 h of B.juncea feeding,26 miRNAs and 433 mRNAs were differentially expressed at 48 h.For the mRNAs,the up-regulated genes were mostly enriched in detoxification enzymes(cytochrome P450,esterase,glutathione^-transferase,uridine diphosphate-glucuronosyl transferase),while the down-regulated genes were mostly enriched in proteinases and immune-related genes.Furthermore,most detoxification enzymes begin to up-regulate at 6 h,while most digestion and immunerelated genes begin to up-or down-regulate at 48 h.Eighteen and 37 differently expressed transcription factors were identified at 6 h and 48 h,which may regulate the functional genes.We acquired 136 and 41 miRNA versus mRNA pairs at 6 h and 48 h,respectively.Some down-regulated and up-regulated miRNAs were predicted to target detoxification enzymes and proteinases,respectively.Real-time quantitative polymerase chain reaction of nine randomly selected miRNAs and 28 genes confirmed the results of RNA-seq.This analyses of miRNA and mRNA transcriptomes provides useful information about the molecular mechanisms of S.litura response to B.juncea.
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