5-Formyluracil targeted biochemical reactions with proteins inhibit DNA replication,induce mutations and interference gene expression in living cells

作     者：Guangrong Zou Kaiyuan Zhang Wei Yang Chaoxing Liu Zhentian Fang Xiang Zhou Guangrong Zou;Kaiyuan Zhang;Wei Yang;Chaoxing Liu;Zhentian Fang;Xiang Zhou

作者机构：College of Chemistry and Molecular SciencesKey Laboratory of Biomedical Polymers of Ministry of EducationThe Institute for Advanced StudiesHubei Province Key Laboratory of Allergy and Immunology Wuhan UniversityWuhan 430072China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2021年第32卷第10期

页      面：3252-3256页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：National Natural Science Foundation of China for their financial support (Nos.91753201  21721005) 

主　　题：5-Formyluracil Biochemicalreaction DNA replication Mutation Gene expression 

摘      要：Covalent DNA–protein cross-links are toxic DNA lesions that interfere with essential biological processes,which can cause serious biological consequences,such as genomic instability and protein misexpression.5-Formyluracil(5 fU) as an important modification in DNA,which is mainly from oxidative damage,exists in a variety of cells and *** have reported that 5 fU mediated DNA–protein conjugates could exist in human cells [Zhou et *** Chem.2(2020) 54–63].We now aimed to explore its potential biological effects in vitro and in *** this paper,we firstly reported that 5 fU intermediated DNA–peptide or DNA–protein conjugates(both were called DPCs) could inhibit different polymerases bypass or cause *** we further investigated the functional impacts caused by 5 fU-mediated DPCs,which appeared in different gene expression components [in the promoter sequence or 50-untranslated regions(UTR)].These results together may contribute to a broader understanding of DNA–protein interactions as well as the biological functions associated with 5 fU.