Monitoring intracellular pH fluctuation with an excited-state intramolecular proton transfer-based ratiometric fluorescent sensor

作     者：Bin Feng Yingli Zhu Jiaxin Wu Xueyan Huang Rong Song Liu Huang Xueping Feng Wenbin Zeng Bin Feng;Yingli Zhu;Jiaxin Wu;Xueyan Huang;Rong Song;Liu Huang;Xueping Feng;Wenbin Zeng

作者机构：Xiangya School of Pharmaceutical SciencesCentral South UniversityChangsha 410013China The Molecular Imaging Research CenterCentral South UniversityChangsha 410013China Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic DiseasesCentral South UniversityChangsha 410013China Xiangya HospitalCentral South UniversityChangsha 410013China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2021年第32卷第10期

页      面：3057-3060页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 071009[理学-细胞生物学] 09[农学] 070302[理学-分析化学] 0703[理学-化学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：the financial supports from National Natural Science Foundation of China (Nos.81971678 and 81671756) the Innovation Fund for Postgraduate Students of Central South University (No.2019zzts1019) 

主　　题：Excited-state intramolecular proton transfer Intracellular pH Sulfonamide Ratiometric sensor Fluorescent imaging 

摘      要：Intracellular pH is a key parameter related to various biological and pathological *** this study,a ratiometric pH fluorescent sensor ABTT was developed harnessing the amino-type excited-state intramolecular proton transfer(ESIPT) *** on whether the ESIPT proceeds normally or not,ABTT exhibited the yellow fluorescence in acidic media,or cyan fluorescence in basic *** to the variation,ABTT behaved as a promising sensor which possessed fast and reversible response to pH change without interference from the biological substances,and exported a steady ratiometric signal(I_(478)/I_(546)).Moreover,due to the ESIPT effect,large Stokes shift and high quantum yield were also exhibited in ***,ABTT was applied for monitoring the pH changes in living cells and visualizing the pH fluctuations under oxidative stress *** results elucidated great potential of ABTT in understanding pH-dependent physiological and pathological processes.