Tetrahydroxystilbene glucoside protects against sodium azide-induced mitochondrial dysfunction in human neuroblastoma cells

作     者：Ru-yi Zhang Xu Zhang Lan Zhang Yan-chuan Wu Xue-jing Sun Lin Li Ru-yi Zhang;Xu Zhang;Lan Zhang;Yan-chuan Wu;Xue-jing Sun;Lin Li

作者机构：Department of PharmacyXuanwu Hospital of Capital Medical UniversityBeijing Engineering Research Centre for Nerve System DrugsBeijing Institute for Brain DisordersKey Laboratory for Neurodegenerative Diseases of Ministry of EducationBeijing 100053China Department of Central LaboratoryXuanwu Hospital of Capital Medical UniversityBeijing 100053China Department of HaematologyXuanwu Hospital of Capital Medical UniversityBeijing 100053China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2021年第13卷第2期

页      面：255-260页

学科分类：1002[医学-临床医学] 100203[医学-老年医学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (Nos.81273498, 81341087) National Science and Technology Major Project of China (No.2015ZX09101-016) 

主　　题：Alzheimer’s disease mitochondria tetrahydroxystilbene glucoside sodium azide 

摘      要：Objective:Mitochondrial dysfunction is evident in the early stage of Alzheimer s disease(AD).Therefore development of drugs that protect mitochondrial function is a promising strategy for *** present work was to investigate the effects of 2,3,5,4 -Tetrahydroxystilbene-2-O-β-d-glucosides(TSG) on a mitochondrial dysfunction cell model induced by sodium azide and elucidate the underlying ***:Mitochondrial membrane potential(MMP) was detected by a fluorescence *** adenosine triphosphate(ATP) level was measured using a firefly luciferase-based *** oxygen species(ROS) was detected using dichlorofluorescin diacetate(DCFH-DA).The expression levels of Bcl-2 and Bax were measured by Western blotting *** cytometry was utilized to measure ***:Pretreatment of TSG(25-200 μmol/L) for 24 h significantly elevated MMP and ATP content,reduced ROS level and Bax/Bcl-2 ratio,and inhibited apoptosis in SH-SY5 Y cells exposed to sodium ***:These results suggest that TSG protects SH-SY5 Y cells against sodium azide-induced mitochondrial dysfunction and *** findings are helpful to understand the protective effect of TSG on mitochondria,which are involved in the early stage of AD.