Iguratimod promotes transformation of mononuclear macrophages in elderly patients with rheumatoid arthritis by nuclear factor-κB pathway

作     者：Sha Liu Li-Ping Song Rong-Bin Li Le-Heng Feng Hui Zhu 

作者机构：Department of RheumatismThe First Hospital of QiqiharAffiliated Qiqihar Hospital of Southern Medical UniversityQiqihar 161005Heilongjiang ProvinceChina 

出 版 物：《World Journal of Clinical Cases》 (世界临床病例杂志)

年 卷 期：2021年第9卷第10期

页      面：2181-2191页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Rheumatoid arthritis Iguratimod Macrophage Polarity Nuclear factor-κB 

摘      要：BACKGROUND The role of macrophages in rheumatoid arthritis(RA)and its mechanism have attracted much attention in RA *** accumulate in the synoviums of RA,and the proportion of M1 type pro-inflammatory macrophages is higher than that of M2 type anti-inflammatory macrophages,leading to the secretion of inflammatory molecules and the aggravation of inflammatory reaction,which has made macrophages a potential target of RA *** is a kind of cyclo-oxygenase-2 inhibitor that affects macrophage *** is speculated that its anti-inflammatory and anti-rheumatic effects may be related to the regulation of macrophage M1/M2 *** To investigate the effects of Iguratimod on the polarity of mononuclear macrophages in elderly patients with *** Elderly patients with RA and joint effusion were selected,including 10 men and 25 women,with an average age of 66.37±4.42 *** were treated with oral administration of 25 mg Iguratimod(Iremod,State Food and Drug Administration Approval No.H20110084)twice daily for 12 *** Activity Score 28 and Health Assessment Questionnaire score were collected according to the disease severity before and after *** blood and joint effusion fluid were collected,mononuclear macrophages were extracted and expression of cell surface markers CD86,CD64,CD163,and CD206 was analyzed by flow *** concentration of inflammatory factors interleukin(IL)-6,IL-1β,transforming growth factor-β,and IL-4 in the joint effusion fluid was analyzed by enzyme-linked immunosorbent *** of mononuclear cells inhibitor of nuclear factor-κB(IκB)and phosphorylated IκB in peripheral blood was analyzed by western *** Disease Activity Score 28 score and Health Assessment Questionnaire score of patients treated with Iguratimod decreased *** percentage of cell surface markers CD86 and CD64 decreased significantly,and the percentage of CD163 and CD206 increased signifi