Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate(MVA)and lycopene expression pathways
作者机构：State Key Laboratory of Bioreactor Engineering East China University of Science and Technology130 Meilong RoadShanghai200237PR China Institute of Environmental Sciences and EngineeringSchool of Civil and Environmental Engineering National University of Sciences and Technology(NUST)Sector H-12Islamabad44000Pakistan Beijing Institute of Biotechnology20 Dongda StreetBeijing100071PR China
出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)
年 卷 期：2021年第6卷第2期
页 面：85-94页
核心收录：
学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 083201[工学-食品科学]
基 金：supported by grants from the National Key Research and Development Program of China(2018YFA0900300 and 2019YFA090141) the National Science and Technology Major Project(2017ZX07402003)
主 题：Escherichia coli Lycopene biosynthesis Rational model of expression pathways Integrated MVA pathway Fermentation
摘 要：The stability and high productivity of heterogeneous terpenoid production in Escherichia coli expression system is one of the most key issues for its large scale *** the current study on taking lycopene biosynthesis as an example,an integrated Escherichia coli system has been generated successfully,which resulted into stable and high lycopene *** this process,two modules of mevalonate(MVA)pathway and one module of lycopene expression pathway were completely integrated in the ***,the copy number and integrated position of three modules of heterologous pathways were rationally ***,a strain DH416 equipped with heterogeneous expression pathways through chromosomal integration was efficiently derived from parental strain *** evolving DH416 strain efficiently produced the lycopene level of 1.22 g/L(49.9 mg/g DCW)in a 5 L fermenter with mean productivity of 61.0 mg/L/***,the integrated strain showed more genetic stability than the plasmid systems after successive 21st passage.
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