Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling

作     者：Yung Hyun Choi Yung Hyun Choi

作者机构：Anti-Aging Research CenterDong-eui UniversityBusan 47340Republic of Korea Department of BiochemistryCollege of Korean MedicineDong-eui UniversityBusan 47227Republic of Korea 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2021年第11卷第5期

页      面：222-230页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Honokiol ROS DNA damage Apoptosis AMPK 

摘      要：Objective:To investigate the effect of honokiol on oxidative damage in HaCaT human ***:HaCaT cells were exposed to hydrogen peroxide(H_(2)O_(2)),following pretreatment with various concentrations of *** alleviating effects of honokiol on HaCaT cell viability and cell death,reactive oxygen species(ROS)production,DNA damage,mitochondrial dynamics,and inhibition of adenosine triphoaphate production against H_(2)O_(2)were *** blotting analysis was used to analyze the expression levels of specific ***:Honokiol suppressed H_(2)O_(2)-induced cytotoxicity and DNA damage by blocking abnormal ROS *** also prevented apoptosis by inhibiting loss of mitochondrial membrane potential and release of cytochrome c from the mitochondria into the cytosol,decreasing the Bax/Bcl-2 ratio,and reducing the activity of caspase-3 in H_(2)O_(2)-stimulated HaCaT *** addition,honokiol attenuated H_(2)O_(2)-induced reduction of adenosine triphosphate content,and activation of AMP-activated protein kinase(AMPK)was markedly promoted by honokiol in H_(2)O_(2)-stimulated ***,the anti-apoptosis and anti-proliferative activity of honokiol against H_(2)O_(2)was further enhanced by adding an activator of AMPK,indicating that honokiol activated AMPK in HaCaT keratinocytes to protect against oxidative ***:The present results indicate that honokiol may be useful as a potential therapeutic agent against various oxidative stress-related skin diseases.