Responses of ammonia-oxidizing microorganisms to biochar and compost amendments of heavy metals-polluted soil

作     者：Mingyue Li Jiachao Zhang Xiao Yang Yaoyu Zhou Lihua Zhang Yuan Yang Lin Luo Qingyun Yan Mingyue Li;Jiachao Zhang;Xiao Yang;Yaoyu Zhou;Lihua Zhang;Yuan Yang;Lin Luo;Qingyun Yan

作者机构：College of Resources and EnvironmentHunan Agricultural UniversityChangsha 410128China Environmental Microbiomics Research CenterSchool of Environmental Science and EngineeringSouthern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Sun Yat-sen UniversityGuangzhou 510006China Korea Biochar Research CenterO-Jeong Eco-Resilience Institute(OJERI)&Division of Environmental Science and Ecological EngineeringKorea UniversitySeoul 02841Republic of Korea 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2021年第33卷第4期

页      面：263-272页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 082803[工学-农业生物环境与能源工程] 07[理学] 08[工学] 0828[工学-农业工程] 0713[理学-生态学] 

基　　金：supported by the Hunan Provincial Key Research and Development Project(Nos.2019WK2031 and 2017SK2351) the National Natural Science Foundation of China(No.51408219) the Natural Science Foundation of Hu-nan Province(No.2020JJ5259) the Outstanding Youth Fund Project of the Hunan Education Department(No.18B094)。 

主　　题：Biochar Compost Heavy metal Ammonia-oxidizing community Ammonia monooxygenase activity 

摘      要：Heavy metal pollution affects soil ecological function.Biochar and compost can effectively remediate heavy metals and increase soil nutrients.The effects and mechanisms of biochar and compost amendments on soil nitrogen cycle function in heavy-metal contaminated soils are not fully understood.This study examined how biochar,compost,and their integrated use affected ammonia-oxidizing microorganisms in heavy metal polluted soil.Quantitative PCR was used to determine the abundance of ammonia-oxidizing archaea(AOA)and bacteria(AOB).Ammonia monooxygenase(AMO)activity was evaluated by the enzymelinked immunosorbent assay.Results showed that compost rather than biochar improved nitrogen conversion in soil.Biochar,compost,or their integrated application significantly reduced the effective Zn and Cd speciation.Adding compost obviously increased As and Cu effective speciation,bacterial 16 S rRNA abundance,and AMO activity.AOB,stimulated by compost addition,was significantly more abundant than AOA throughout remediation.Correlation analysis showed that AOB abundance positively correlated with NO_(3)^(-)-N(r=0.830,P0.01),and that AMO activity had significant correlation with EC(r=-0.908,P0.01)and water-soluble carbon(r=-0.868,P0.01).Those seem to be the most vital factors affecting AOB community and their function in heavy metal-polluted soil remediated by biochar and compost.