Comparison of the protective effects of resveratrol and pterostilbene against intestinal damage and redox imbalance in weanling piglets

作     者：Hao Zhang Yanan Chen Yueping Chen Shuli Ji Peilu Jia Yue Li Tian Wang Hao Zhang;Yanan Chen;Yueping Chen;Shuli Ji;Peilu Jia;Yue Li;Tian Wang

作者机构：College of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095JiangsuChina Postdoctoral Research Station of Clinical Veterinary MedicineCollege of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095JiangsuChina Shanghai Key Laboratory of Veterinary BiotechnologyShanghai 200240China Postdoctoral Research Station of Food Science and EngineeringCollege of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095JiangsuChina Institute of Animal ScienceJiangsu Academy of Agricultural SciencesNanjing 210014China. 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2020年第11卷第4期

页      面：1204-1219页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 090502[农学-动物营养与饲料科学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：offered by from the National Natural Science Foundation of China (No. 31802094) the Fundamental Research Funds for the Central Universities (No. KJQN201934) the Natural Science Foundation of Jiangsu Province (No. BK20180531) the Postdoctoral Research Foundation of China (No. 2018 M632320 and 2019 T120436) the Open Project of Shanghai Key Laboratory of Veterinary Biotechnology (No.klab201710)。 

主　　题：Intestinal injury Oxidative damage Piglet Pterostilbene Resveratrol Weaning stress 

摘      要：Background: Evidence indicates that early weaning predisposes piglets to intestinal oxidative stress and increases the risk of intestinal dysfunction;however, there are minimal satisfactory treatment strategies for these conditions.This study investigated the potential of resveratrol and its analog, pterostilbene, as antioxidant protectants for regulating intestinal morphology, barrier function, and redox status among weanling piglets.Methods: A total of 144 piglets were selected at 21 days of age and randomly allocated into one of four treatment groups, each of which included six replicates. Piglets in a sow-reared control group were suckling normally between ages 21 and 28 days, while those in weaned groups were fed a basal diet, supplemented with either 300 mg/kg of resveratrol or with 300 mg/kg of pterostilbene. Parameters associated with intestinal injury and redox status were analyzed at the end of the feeding trial.Results: Early weaning disrupted the intestinal function of young piglets, with evidence of increased diamine oxidase activity and D-lactate content in the plasma, shorter villi, an imbalance between cell proliferation and apoptosis, an impaired antioxidant defense system, and severe oxidative damage in the jejunum relative to suckling piglets. Feeding piglets with a resveratrol-supplemented diet partially increased villus height(P = 0.056) and tended to diminish apoptotic cell numbers(P = 0.084) in the jejunum compared with those fed a basal diet. Similarly, these beneficial effects were observed in the pterostilbene-fed piglets. Pterostilbene improved the feed efficiency of weanling piglets between the ages of 21 and 28 days;it also resulted in diminished plasma diamine oxidase activity and D-lactate content relative to untreated weaned piglets(P 0.05). Notably, pterostilbene restored jejunal antioxidant capacity, an effect that was nearly absent in the resveratrol-fed piglets. Pterostilbene reduced the malondialdehyde and 8-hydroxy-2′-deoxyguanosine contents of jejunal mucosa possibly through its regulatory role in facilitating the nuclear translocation of nuclear factor erythroid-2-related factor 2 and the expression levels of NAD(P)H quinone dehydrogenase 1 and superoxide dismutase 2(P 0.05).Conclusions: The results indicate that pterostilbene may be more effective than its parent compound in alleviating early weaning-induced intestinal damage and redox imbalance among young piglets.