Establishment of a Reverse Genetic System of Severe Fever with Thrombocytopenia Syndrome Virus Based on a C4 Strain

作     者：Mingyue Xu Bo Wang Fei Deng Hualin Wang Manli Wang Zhihong Hu Jia Liu Mingyue Xu;Bo Wang;Fei Deng;Hualin Wang;Manli Wang;Zhihong Hu;Jia Liu

作者机构：State Key Laboratory of Virology and National Virus Resource CenterWuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China University of the Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2021年第36卷第5期

页      面：958-967页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(No.31900146 Open Research Fund Program of the State Key Laboratory of Virology of China(No.2020IOV003) Team project of Health Commission of Hubei Province(WJ2019C003) 

主　　题：Bunyavirus Severe fever with thrombocytopenia syndrome virus(SFTSV) Minigenome Reverse genetic system T7 polymerase C4 strain 

摘      要：Severe fever with thrombocytopenia syndrome virus(SFTSV)is an emerging tick-borne bunyavirus that causes hemorrhagic fever-like disease(SFTS)in humans with a case fatality rate up to 30%.To date,the molecular biology involved in SFTSV infection remains *** are seven major genotypes of SFTSV(C1-C4 and J1-J3)and previously a reverse genetic system was established on a C3 strain of ***,we reported successfully establishment of a reverse genetics system based on a SFTSV C4 ***,we obtained the 5’-and 3’-terminal untranslated region(UTR)sequences of the Large(L),Medium(M)and Small(S)segments of a laboratory-adapted SFTSV C4 strain through rapid amplification of cDNA ends analysis,and developed functional T7 polymerase-based L-,M-and S-segment minigenome ***,fulllength cDNA clones were constructed and infectious SFTSV were recovered from co-transfected *** infectivity,growth kinetics,and viral protein expression profile of the rescued virus were compared with the laboratory-adapted *** formation assay showed that the size and morphology of the foci formed by the rescued SFTSV were indistinguishable with the laboratory-adapted ***,one-step growth curve and nucleoprotein expression analyses revealed the rescued virus replicated less efficiently than the laboratory-adapted *** analysis indicated that the difference may be due to the mutations in the laboratory-adapted strain which are more prone to cell *** results help us to understand the molecular biology of SFTSV,and provide a useful tool for developing vaccines and antivirals against SFTS.