Targeted combination therapy for glioblastoma by co-delivery of doxorubicin,YAP-siRNA and gold nanorods

作     者：Lihuang Li Qiuyan Guo Yanxiu Liu Mindan Lu Jun Yang Yunlong Ge Qiang Zhang Benqiang Sun Xiumin Wang Li Liang-cheng Lei Ren Lihuang Li;Qiuyan Guo;Yanxiu Liu;Mindan Lu;Jun Yang;Yunlong Ge;Qiang Zhang;Benqiang Sun;Xiumin Wang;Li Liang-cheng;Lei Ren

作者机构：Key Laboratory of Biomedical Engineering of Fujian Province University/Research Center of Biomedical Engineering of XiamenDepartment of BiomaterialsCollege of MaterialsXiamen UniversityXiamen 361005China School of Pharmaceutical SciencesFujian Provincial Key Laboratory of Innovative Drug Target ResearchXiamen UniversityXiamen 361102China Department of NeurosurgeryXiang’an Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361102China Stomatological Hospital of Xiamen Medical CollegeXiamen 361105China State Key Lab of Physical Chemistry of Solid SurfacesXiamen UniversityXiamen 361005China 

出 版 物：《Journal of Materials Science & Technology》 (材料科学技术（英文版）)

年 卷 期：2021年第63卷第4期

页      面：81-90页

核心收录：

学科分类：07[理学] 070205[理学-凝聚态物理] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 0817[工学-化学工程与技术] 0806[工学-冶金工程] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0703[理学-化学] 0802[工学-机械工程] 0702[理学-物理学] 10[医学] 0801[工学-力学（可授工学、理学学位）] 

基　　金：supported by National Natural Science Foundation of China(U190420008 31870994)。 

主　　题：doxorubicin glioblastoma simultaneous 

摘      要：The combination of brain targeting drug delivery systems and multi-modal intervention pose a promising therapeutic approach for glioblastoma therapy.In this study,we developed an angiopep-2 peptide modified cationic liposome loaded with doxorubicin,YAP-siRNA and gold nanorods(D/R@Ang2-Lip+Au)simultaneously,which has high encapsulating efficiency for doxorubicin(95.4%)and effective binding of siRNA at N/P ratio of 20:1.The fluorescence imaging and flow cytometry analysis revealed high cellular uptake of D/R@Ang2-Lip+Au.Real-time quantitative polymerase chain reaction and western blot analysis indicated that D/R@Ang2-Lip+Au could effectively inhibit the expression of YAP protein.In vitro and in vivo studies showed that D/R@Ang2-Lip+Au had the ability to target glioblastoma cells,and achieved better anti-proliferative effects compared with non-targeted D/R@Lip+Au.Moreover,in vivo experiment demonstrated that D/R@Ang2-Lip+Au was able to cross the blood-brain barrier,and combination therapy could significantly inhibit tumor growth.Therefore,the multifunctional D/R@Ang2-Lip+Au might provide a novel approach for effectively delivery of DOX,YAP-siRNA and AuNRs into the glioblastoma cells simultaneously and exerting synergistic therapeutic effects.