Effects of HDAC4 on IL-1β-induced matrix metalloproteinase expression regulated partially through the WNT3A/β-catenin pathway

作     者：Qi Ning Ye-Hua Gan Rui-Rui Shi Juan-Hong Meng Ning Qi;Gan Ye-Hua;Shi Rui-Rui;Meng Juan-Hong

作者机构：Department of Oral and Maxillofacial SurgeryPeking University School and Hospital of StomatologyBeijing 100081China Central LaboratoryPeking University School and Hospital of StomatologyBeijing 100081China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2021年第134卷第8期

页      面：963-970页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：the National Natural Science Foundation of China(No.31671506) 

主　　题：Histone deacetylase 4 Matrix metalloproteinase 13 Matrix metalloproteinase 3 Osteoarthritis WNT3A 

摘      要：Background::Histone deacetylase 4(HDAC4)regulates chondrocyte hypertrophy and bone *** aim of the present study was to explore the effects of HDAC4 on Interleukin 1 beta(IL-1β)-induced chondrocyte extracellular matrix degradation and whether it is regulated through the WNT family member 3A(WNT3A)/β-catenin signaling ***::Primary chondrocytes(CC)and human chondrosarcoma cells(SW1353 cells)were treated with IL-1βand the level of HDAC4 was assayed using Western ***,HDAC4 expression in the SW1353 cells was silenced using small interfering RNA to detect the effect of HDAC4 knockdown on the levels of matrix metalloproteinase 3(MMP3)and MMP13 induced by IL-1β.After transfection with HDAC4 plasmids,the overexpression efficiency was examined using Real-time quantitative polymerase chain reaction(qRT-PCR)and the levels of MMP3 and MMP13 were assayed using Western *** incubation with IL-1β,the translocation ofβ-catenin into the nucleus was observed using immunofluorescence staining in SW1353 cells to investigate the activation of the WNT3A/β-catenin signaling ***,treatment with WNT3A and transfection with glycogen synthase kinase 3 beta(GSK3β)plasmids were assessed for their effects on HDAC4 levels using Western ***::IL-1βdownregulated HDAC4 levels in chondrocytes and SW1353 ***,HDAC4 knockdown increased the levels of MMP3 and MMP13,which contributed to the degradation of the extracellular *** of HDAC4 inhibited IL-1β-induced increases in MMP3 and ***-1βupregulated the levels of WNT3A,and WNT3A reduced HDAC4 levels in SW1353 ***-3βrescued IL-1β-induced downregulation of HDAC4 in SW1353 ***::HDAC4 exerted an inhibitory effect on IL-1β-induced extracellular matrix degradation and was regulated partially by the WNT3A/β-catenin signaling pathway.