The hist one varia nt SI_H2A.Z regulates carote noid biosynthesis and gene expression during tomato fruit ripening

作     者：Xuedong Yang Xuelian Zhang Youxin Yang Hui Zhang Weimin Zhu Wen-Feng Nie Xuedong Yang;Xuelian Zhang;Youxin Yang;Hui Zhang;Weimin Zhu;Wen-Feng Nie

作者机构：Eha nghai Key Lab oratory of Pro tected Hor ticult ural Tech no logyHor ticult ure Research InstituteShanghai Academy of Agricultural Sciences201403 ShanghaiChina Department of HorticultureCollege of AgronomyJiangxi Agricultural University330045 NanchangJiangxiChina Department of HorticultureCollege of Horticulture and Plant ProtectionYangzhou University225009 YangzhouJiangsuChina 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2021年第8卷第1期

页      面：979-995页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：the National Key R&D Program of China(2016YFD0101703 and 2017YFD0101902) the Natural ScienCe Foundation of Jiangsu Province(BK20200948) the National Nature ScienCe Foundation of China(32002046) 

主　　题：H2A.Z utilized programming 

摘      要：The conserved histone variant H2A.Z is essential for transcriptional regulation;defense responses;and various biological processes in plants,such as growth,development,and ***,little is known about how H2A.Z affects the developmental process and ripening of tomato ***,we utilized the CRISPR/Cas9 gene-editing system to generate a sl_hta9 sl_hta11 double-mutant,designated sl_h2az,and found that these two mutations led to a signi fi cant reduction in the fresh weight of tomato *** messenger RNA(mRNA)-seq results showed that dysfunction of SI_H2A.Z has profound effects on the reprogramming of genome-wide gene expression at different developmental stages of tomato fruits,indicating a ripening-dependent correlation between SI_H2A.Z and gene expression regulation in tomato *** addition,the expression of three genes,SIPSY1,SlPDS,and SlVDE,encoding the key enzymes in the biosynthesis pathway of carotenoids,was signi fi cantly upregulated in the later ripening stages,which was consistent with the increased contents of carotenoids in sl_h2a.z double-mutant ***,our study reveals a role of SI_H2A.Z in the regulation of carotenoids and provides a resource for the study of SI_H2A.Z-dependent gene expression ***,our results provide a link between epigenetic regulation via histone variants and fruit development,suggesting a conceptual framework to understand how histone variants regulate tomato fruit quality.