Transcriptome analysis of molecular mechanisms underlying facial nerve injury repair in rats

作     者：Qian-Qian Cao Shuo Li Yan Lu Di Wu Wei Feng Yong Shi Lu-Ping Zhang Qian-Qian Cao;Shuo Li;Yan Lu;Di Wu;Wei Feng;Yong Shi;Lu-Ping Zhang

作者机构：Key Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationCo-innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina Department of OtolaryngologyAffiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of OtolaryngologyHead and Neck SurgeryEyeEarNose and Throat HospitalFudan UniversityShanghaiChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2021年第16卷第11期

页      面：2316-2323页

核心收录：

学科分类：1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100204[医学-神经病学] 10[医学] 

主　　题：Akr1b8 cell proliferation facial nerve injury Gene-Act Networks inflammatory response RNA-Seq Schwann cells transcriptomics analysis 

摘      要：Although the transcriptional alterations inside the facial nucleus after facial nerve injury have been well studied,the gene expression changes in the facial nerve trunk after injury are still *** this study,we established an adult rat model of facial nerve crush injury by compressing the right lateral extracranial nerve *** sequencing,differential gene expression analysis,and cluster analysis of the injured facial nerve trunk were performed,and 39 intersecting genes with significant variance in expression were *** Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway analyses of the 39 intersecting genes revealed that these genes are mostly involved in leukocyte cell-cell adhesion and phagocytosis and have essential roles in regulating nerve *** real-time polymerase chain reaction assays were used to validate the expression of pivotal ***,nine pivotal genes that contribute to facial nerve recovery were identified,including Arhgap30,Akr1b8,C5ar1,Csf2ra,Dock2,Hcls1,Inpp5d,Sla,and *** Schwann cells were isolated from the sciatic nerve of neonatal *** knocking down Akr1b8 in Schwann cells with an Akr1b8-specific small interfering RNA plasmid,expression levels of monocyte chemoattractant protein-1 and interleukin-6 were decreased,while cell proliferation and migration were not obviously *** findings suggest that Akr1b8 likely regulates the interaction between Schwann cells and macrophages through regulation of cytokine expression to promote facial nerve *** study is the first to reveal a transcriptome change in the facial nerve trunk after facial nerve injury,thereby revealing the potential mechanism underlying repair of facial nerve *** study was approved by the Animal Ethics Committee of Nantong University,China in 2018(approval No.S20180923-007).