Lentinula edodes extract inhibits matrix metalloproteinase expression and increases typeⅠprocollagen expression via the p38 MAPK/c-Fos signaling pathway in ultraviolet A and B-irradiated HaCaT keratinocytes

作     者：Jung Im Lee Jung Hwan Oh Fatih Karadeniz So Young Park Hye Ran Kim Hyun Jin Jo Kyung Im Jung Byung-Jin Jeon Chang-Suk Kong Jung Im Lee;Jung Hwan Oh;Fatih Karadeniz;So Young Park;Hye Ran Kim;Hyun Jin Jo;Kyung Im Jung;Byung-Jin Jeon;Chang-Suk Kong

作者机构：Marine Biotechnology Center for Pharmaceuticals and FoodsSilla UniversityBusan 46958Korea Department of Food and NutritionCollege of Medical and Life SciencesSilla UniversityBusan 46958Korea Ocean Fisheries and Bio CenterBusan technoparkBusan 46048Korea 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2021年第11卷第4期

页      面：164-173页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by the Ministry of Trade Industry&Energy Korea Institute for Advancement of Technology and Busan Institute For Regional Program Evaluation through the Encouragement Program for the Social and Economic Innovation Growth(project number P0008724) 

主　　题：Lentinula edodes Ultraviolet Matrix metalloproteinases TypeⅠprocollagen Ha Ca T 

摘      要：Objective:To determine the effect of Lentinula edodes extract on ultraviolet(UV)A and UVB-induced changes in matrix metalloproteinase(MMP)and type I procollagen expression using human immortalized HaCaT ***:Lentinula edodes ethanol extract(LEE)was obtained by extraction with 80%ethanol for 4 h at 80℃.Effect of LEE on UVinduced alteration on the expression and production of MMPs and type I procollagen in keratinocytes was investigated using ELISA,RT-PCR,and Western blotting *** determine the underlying mechanism of LEE-mediated effects,mitogen-activated protein kinase(MAPK)and activator protein 1 signaling pathways were analysed by Western blotting ***:LEE significantly inhibited the expression of MMP-1 and MMP-9 and increased the expression of type I procollagen in UVA and UVB-irradiated HaCaT *** phosphorylation levels of p38 were significantly inhibited by LEE whereas it did not affect c-Jun N-terminal kinase and extracellular signal-regulated kinase *** of p38 phosphorylation was also accompanied by downregulation of UVA and UVB-induced increase in ***:LEE effectively inhibits the expression of MMP-1 and MMP-9 and increases type I procollagen production through the p38 MAPK/c-Fos signaling pathway in UVA and UVB-irradiated HaCaT *** findings suggest that Lentinula edodes may be developed as a cosmetic material to suppress UV exposuremediated skin aging.