Antioxidants vitamin E and C attenuate hepatic fibrosis in biliary-obstructed rats

作     者：Ali Riza Soylu Nurettin Aydogdu Umit Nusret Basaran Semsi Altaner Orhan Tarcin Nursal Gedik Hasan Umit Ahmet Tezel Gulbin Dokmeci Huseyin Baloglu Mevlut Ture Kemal Kutlu Kadir Kaymak 

作者机构：Division of Gastroenterology Trakya University Edirne Turkey Department of Physiology Trakya University Edirne Turkey Department of Pediatric Surgery Trakya University Edirne Turkey Department of Pathology Trakya University Edirne Turkey Division of Gastroenterology Gumussuyu Military Hospital Istanbul Turkey Department of Biochemistry Kas?mpasa Naval Hospital Istanbul Turkey Department of Pathology Haydarpasa GATA/ Education Hospital Istanbul Turkey Department of Biostatistics Trakya University Edirne Turkey 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第42期

页      面：6835-6841页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Trakya University Research Fund. TUBAP No. 548 

主　　题：Hepatic fibrosis Biliary obstruction Collagen Vitamin E Vitamin C 

摘      要：AIM： To investigate whether antioxidants vitamin E and C can retard development of hepatic fibrosis in the bilian/obstructed rats. METHODS： Fifty Wistar albino rats were randomly assigned to 5 groups （10 rats in each）. Bile duct was ligated in 40 rats and they were treated as follows： group vitC, vitamin C 10 mg/kg sc daily; group vitE, vitamin E 15 mg/kg sc daily; group vitEC, both of the vitamins; bile duct-ligated （BDL, control） group, physiological saline sc. The fifth group was assigned to sham operation. At the end of fourth week, the rats were decapitated, and hepatic tissue biochemical collagen content and collagen surface area were measured. Hepatic tissue specimens were histopathologically evaluated according to Scheuer system. Serum hyaluronate levels were measured by ELISA method. RESULTS： Despite being higher than sham group, hepatic collagen level was significantly decreased in each of the vitC, vitE and vitEC groups （32.7 ± 1.2, 33.8 ± 2.9, 36.7 ± 0.5 ）μg collagen/mg protein, respectively） compared to BDL （48.3 ± 0.6 mg collagen/g protein） （P 〈 0.001 for each vitamin group）. Each isolated vitamin C, isolated vitamin E and combined vitamin E/C supplementation prevented the increase in hepatic collagen surface density （7.0% ± 1.1%, 6.2% ± 1.7%, 12.3% ± 2.0%, respectively） compared to BDL （17.4% ± 5.6%） （P 〈 0.05 for each）. The same beneficial effect of vitamin C, vitamin E and combined vitamin E/C treatment was also observed on the decrease of serum hyaluronate levels compared to BDL group （P 〈 0.001）. The relative liver and spleen weights, serum transaminases, cholestatic enzymes, bilirubins and histopathological inflammation scores were not different between the antioxidant treatment groups and the control. However, fibrosis staging scores were obviously reduced only in the vitamin E/C combination group （vit EC： 2.4 ± 0.8 vs BDL： 3.1 ± 0.7; P 〈 0.05）. CONCLUSION： Each antioxidant vitamin E, vitamin C and their combination retard hepatic fibrosis in bi