Effects of full replacement of dietary fishmeal with insect meal from Tenebrio molitor on rainbow trout gut and skin microbiota

作     者：Genciana Terova Elisabetta Gini Laura Gasco Federico Moroni Micaela Antonini Simona Rimoldi Genciana Terova;Elisabetta Gini;Laura Gasco;Federico Moroni;Micaela Antonini;Simona Rimoldi

作者机构：Department of Biotechnology and Life SciencesUniversity of InsubriaVia J.H.Dunant321100 VareseItaly Department of AgriculturalForest and Food SciencesUniversity of TurinLargo P.Braccini 2-10095 GrugliascoTorinoItaly 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2021年第12卷第3期

页      面：1042-1055页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 09[农学] 

基　　金：This research was partially funded by AGER,Network Foundation,Project Fine Feed for Fish(4F) Rif.No.2016-01-01 This work was also co-funded by the EU Horizon 2020 AquaIMPACT(Genomic and nutritional innovations for genetically superior farmed fish to improve efficiency in European aquaculture),number:818367 

主　　题：Aquaculture Circular economy Gut microbiome Insect meal Metagenome Next-generation sequencing Rainbow trout Skin microbiome Tenebrio molitor 

摘      要：Background:Aquaculture must continue to reduce dependence on fishmeal(FM)and fishoil in feeds to ensure sustainable sector ***,the use of novel aquaculture feed ingredients is *** this regard,insects can represent a new world of sustainable and protein-rich ingredients for farmed fish ***,we investigated the effects of full replacement of FM with Tenebrio molitor(TM)larvae meal in the diet of rainbow trout(Oncorhynchus mykiss)on fish gut and skin ***:A feeding trial was conducted with 126 trout of about 80 g mean initial weight that were fed for 22 weeks with two isonitrogenous,isolipidic,and isoenergetic extruded experimental *** defatted TM meal was included in one of the diets to replace 100%(TM 100)of FM,whereas the other diet(TM 0)was without *** analyse the microbial communities,the Illumina MiSeq platform for sequencing of 16S rRNA gene and Qiime pipeline were used to identify bacteria in the gut and skin mucosa,and in the ***:The data showed no major effects of full FM substitution with TM meal on bacterial species richness and diversity in both,gut mucosa-and skin mucus-associated *** microbiome was dominated by phylum Proteobacteria and especially by Gammaproteobacteria class that constituted approximately half of the bacterial taxa *** two dietary fish groups did not display distinctive features,except for a decrease in the relative abundance of Deefgea genus(family Neisseriaceae)in trout fed with insect *** metagenomic analysis of the gut mucosa indicated that Tenericutes was the most abundant phylum,regardless of the ***,within this phylum,the Mollicutes,mainly represented by Mycoplasmataceae family,were the dominant ***,we observed only a weak dietary modulation of intestinal bacterial *** only changes due to full FM replacement with TM meal were a decreased number of Proteobacteria and a reduced number of taxa assigned to