Metabolic and immunological effects of gut microbiota in leaf beetles at the local and systemic levels
作者机构：State Key Laboratory of Biocatalysis and Enzyme EngineeringSchool of Life SciencesHubei UniversityWuhanChina
出 版 物：《Integrative Zoology》 (整合动物学（英文版）)
年 卷 期：2021年第16卷第3期
页 面：313-323页
核心收录：
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0905[农学-畜牧学] 0906[农学-兽医学] 071005[理学-微生物学] 10[医学]
基 金：This work was funded by the National Key Research and Development Program(2017YFD0600101) Na-tional Natural Science Foundation of China(31971663,31801979) the Young Elite Scientists Sponsorship Program by CAST(2020QNRC001)
主 题：gut bacteria insect immunity nutritional metabolism Plagiodera versicolora RNA-seq
摘 要：Insects’intestinal microbes have profound effects on the host’s physiological traits,which can impact their physiology at both the local(gut)and systemic(body)***,the molecular mechanisms underlying hostmicrobiota interactions,especially in non-model insects,remain ***,tissue-specific transcriptomic analysis has been highlighted as a robust tool in studying host–microbe *** versicolora is a worldwide leaf-eating pest that primarily feeds on willows and *** interplay between gut microflora and this host beetle has yet to be ***,we investigate the effects of the gut microbiota on the body mass of *** larvae,compare the nutrition status of larvae in absence and presence of gut microbiota,and profile gut bacterial loads throughout its developmental larval *** then perform comparative transcriptomic analysis of gut and body tissues in axenic and non-axenic ***,we confirm the expression patterns of representative genes in nutritional metabolism and *** show that weight growth is retarded in conventional larvae,with a concomitant increase of total bacterial load by the 5^(th)development day,and germ-free larvae have a higher glucose content than conventional-reared *** nutritional and immunological analyses indicate that gut bacteria are a burden in the beetle’s larval *** findings elucidate the impacts of gut microbiota on ***,and provide insight into tissue-specific responses to gut microflora in this pest at the genetic level,boosting our understanding of the molecular mechanisms underlying host–microbe interactions in leaf beetles and beyond.
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