Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp.
作者机构：Department of BiochemistryPalackýUniversitySlechtitelu 27CZ-78371 OlomoucCzech Republic Department of BotanyFaculty of SciencePalacky UniversitySlechtitelu27CZ-78371 OlomoucCzech Republic Department of BiochemistryFaculty of ScienceMasaryk UniversityKamenice 753/5CZ-62500 BrnoCzech Republic
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2021年第8卷第1期
页 面：328-342页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 0703[理学-化学] 090401[农学-植物病理学] 0836[工学-生物工程] 0902[农学-园艺学] 090402[农学-农业昆虫与害虫防治] 0834[工学-风景园林学（可授工学、农学学位）]
基 金：Palacky University in Olomouc(IGA_PrF_2020_013)
主 题：Phytophthora cultivated infection
摘 要：Regulation of protein function by reversible S-nitrosation,a post-translational modification based on the attachment of nitroso group to cysteine thiols,has emerged among key mechanisms of NO signalling in plant development and stress responses.S-nitrosoglutathione is regarded as the most abundant low-molecular-weight S-nitrosothiol in plants,where its intracellular concentrations are modulated by S-nitrosoglutathione *** analysed modulations of S-nitrosothiols and protein S-nitrosation mediated by S-nitrosoglutathione reductase in cultivated Solanum lycopersicum(susceptible)and wild Solanum habrochaites(resistant genotype)up to 96h post inoculation(hpi)by two hemibiotrophic oomycetes,Phytophthora infestans and Phytophthora parasitica.S-nitrosoglutathione reductase activity and protein level were decreased by *** and *** infection in both genotypes,whereas protein S-nitrosothiols were increased by *** infection,particularly at 72 hpi related to pathogen biotrophy–necrotrophy *** levels of S-nitrosothiols localised in both proximal and distal parts to the infection site,which suggests together with their localisation to vascular bundles a signalling role in systemic responses.S-nitrosation targets in plants infected with *** identified by a proteomic analysis include namely antioxidant and defence proteins,together with important proteins of metabolic,regulatory and structural *** peroxidase S-nitrosation was observed in both genotypes in parallel to increased enzyme activity and protein level during *** pathogenesis,namely in the susceptible *** results show important regulatory functions of protein S-nitrosation in concerting molecular mechanisms of plant resistance to hemibiotrophic pathogens.
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