The lncRNA APOLO interacts with the transcription factor WRKY42 to trigger root hair cell expansion in response to cold

作     者：Michael Moison Javier Martinez Pacheco Leandro Lucero Camille Fonouni-Farde Johan Rodriguez-Melo Natanael Mansilla Aurelie Christ Jeremie Bazin Moussa Benhamed Fernando Ibanez Martin Crespi Jose M.Estevez Federico Ariel 

作者机构：Instituto de Agrobiotecnología del LitoralUniversidad Nacional del LitoralCONICETFBCB/FHUCCentro Científico Tecnológico CONICET Santa FeColectora Ruta Nacional No 168 km.0Paraje El PozoSanta Fe 3000Argentina Fundación Instituto Leloir and IIBBA-CONICETAv.Patricias Argentinas 435Buenos Aires CP C1405BWEArgentina Instituto de Investigaciones AgrobiotecnológicasCONICETUniversidad Nacional de Río CuartoRío Cuarto 5800Argentina Institute of Plant Sciences Paris-Saclay(IPS2)CNRSINRAUniversity Paris-Saclay and University of Paris Bâtiment 63091192 Gif sur YvetteFrance Centro de Biotecnología Vegetal(CBV)Facultad de Ciencias de la Vida(FCsV)Universidad Andres BelloSantiagoChile and Millennium Institute for Integrative Biology(iBio)SantiagoChile 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2021年第14卷第6期

页      面：937-948页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by grants from ANPCyT(PICT2016-0132 and PICT2017-0066) Instituto Milenio iBio-Iniciativa Cientffica Milenio,MINECON to J.M.E 

主　　题：root hairs long noncoding RNAs APOLO RHD6 WRKY42 cold temperature 

摘      要：Plant long noncoding RNAs(lncRNAs)have emerged as important regulators of chromatin dynamics,impacting on transcriptional programs leading to different developmental *** lncRNA AUXIN-REGULATED PROMOTER LOOP(APOLO)directly recognizes multiple independent loci across the Arabidopsis genome and modulates their three-dimensional chromatin conformation,leading to transcriptional ***,we show that APOLO recognizes the locus encoding the root hair(RH)master regulator ROOT HAIR DEFECTIVE 6(RHD6)and controls RHD6 transcriptional activity,leading to cold-enhanced RH elongation through the consequent activation of the transcription factor gene RHD6-like ***,we demonstrate that APOLO interacts with the transcription factor WRKY42 and modulates its binding to the RHD6 ***42 is required for the activation of RHD6 by low temperatures and WRKY42 deregulation impairs cold-induced RH ***,our results indicate that a novel ribonucleoprotein complex with APOLO and WRKY42 forms a regulatory hub to activate RHD6 by shaping its epigenetic environment and integrate signals governing RH growth and development.