Th17 cells are involved in mouse chronic obstructive pulmonary disease complicated with invasive pulmonary aspergillosis

作     者：Wan-Ru Geng Hang-Yong He Qing Zhang Zhao-Hui Tong Geng Wan-Ru;He Hang-Yong;Zhang Qing;Tong Zhao-Hui

作者机构：Department of Respiratory and Critical Care MedicineBeijing Institute of Respiratory MedicineBeijing Chao-Yang HospitalCapital Medical UniversityBeijing 100020China Department of Respiratory MedicineAffiliated Hospital of Inner Mongolia University for the NationalitiesTongliaoInner Mongolia 028000China Department of Respiratory and Critical Care MedicineThe Second Hospital of Jilin UniversityChangchunJilin 130041China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2021年第134卷第5期

页      面：555-563页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：This study was supported by a grant from the National Natural Science Foundation of China(No.81400003) 

主　　题：T helper cells 17 Chronic obstructive pulmonary disease(COPD) Invasive pulmonary aspergillosis(IPA) 

摘      要：Background:The incidence of chronic obstructive pulmonary disease(COPD)complicated with invasive pulmonary aspergillosis(IPA)has increased in the last two *** mechanism underpinning susceptibility to and high mortality of COPD complicated with IPA is unclear,and the role of T helper cells 17(Th17 cells)in the compound disease remains ***,this study aimed to assess the function of Th17 cells in COPD combined with ***:COPD,IPA,and COPD+IPA mouse models were established in male wild type C57/BL6 *** amounts of Th17 cells and retinoic acid-related orphan receptorsγt(RORyt)were tested by flow ***,serum interleukin(IL)-17 and *** were detected by enzyme-linked immunosorbent assay(ELISA)in the control,COPD,IPA and COPD+IPA *** addition,COPD+IPA was induced in IL-17 knockout(KO)mice,for determining the role of Th17 cells in COPD+***:Compared with the COPD group,the COPD+IPA group showed higher amounts of blood RORyt([35.09±16.12]%vs.[17.92±4.91]%,P=0.02)and serum IL-17(17.96±9.59 pg/mL vs.8.05±4.44 pg/mL,P=0.02),but blood([5.18±1.09]%vs.[4.15±0.87]%,P=0.28)and lung levels of Th17 cells(1.98±0.83]%vs.[2.03±0.98]%,P=0.91),lung levels of RORyt([9.58±6.93]%vs.[9.63±5.98]%,P=0.49)and serum IL-23(51.55±27.82 pg/mL us.68.70±15.20 pg/mL,P=0.15)showed no significant *** with the IPA group,the COPD+IPA group displayed lower amounts of blood([5.18±1.09]%vs.[9.21±3.56]%,P=0.01)and lung Th17 cells([1.98±0.83]%vs.[6.29±1.11]%,P=0.01)and serum IL-23(51.55±27.82 pg/mL vs.154.90±64.60 pg/mL,P=0.01)and IL-17(17.96±9.59 pg/mL uUs.39.81±2.37 pg/mL,P=0.02),while comparable blood([35.09±16.12]%Vs.[29.86±15.42]%,P=0.25)and lung levels of RORγt(9.58±6.93]%VUS,[15.10±2.95]%,P=0.18)were found in these two ***,Aspergillus load in IL-17 KO COPD+IPA mice was almost 2 times that of COPD+IPA mice(1,851,687.69±944,480.43vs.892,958.10±686,808.80,t=2.32,P=0.02).Conclusion:These findings indicate that Th17 cells