Role of circular RNA expression in the pathological progression after spinal cord injury

作     者：Wen-Zhao Wang Jun Li Lei Liu Zheng-Dong Zhang Ming-Xin Li Qin Li Hui-Xu Ma Hai Yang Xiao-Ling Hou Wen-Zhao Wang;Jun Li;Lei Liu;Zheng-Dong Zhang;Ming-Xin Li;Qin Li;Hui-Xu Ma;Hai Yang;Xiao-Ling Hou

作者机构：Department of OrthopedicsNational Clinical Research Center for GeriatricsWest China HospitalSichuan University/West China School of NursingSichuan UniversityChengduSichuan ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2021年第16卷第10期

页      面：2048-2055页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：This study was supported by the National Natural Science Foundation of China,No.81874002(to LL) the Science and Technology Support Project of Sichuan Province of China,Nos.2018SZ0159(to LL),2018SZ0246(to XLH) the Innovation and Entrepreneurship Project of Sichuan Technology Gallery of China,Nos.2019JDRC0100(to JL),2020JDRC0054(to WZW) the National Clinical Research Center for Geriatrics,West China Hospital,Sichuan University,China,Nos.Y2018B22(to LL),Z20192013(to JL) West China Hospital Postdoctoral Research and Development Fund,No.2019HXBH068(to JL) 

主　　题：bioinformatics circRNA/miRNA/mRNA network circular RNA gene KLF4 miR-135b-5p spinal cord injury trauma 

摘      要：Differential expression of non-coding RNA after traumatic spinal cord injury(TSCI)is closely related to the pathophysiological *** purposes of this study were to systematically profile and characterize expression of circular RNA(circRNA)in the lesion epicenter of spinal tissues after TSCI,and predict the structure and potential function of the regulatory circRNA/miRNA ***-eight C57BL/6 mice were randomly and equally assigned to two groups:one subjected to TSCI at T8–10 with an Allen’s drop impactor,and a second subjected to laminectomy without *** cord samples were stained with hematoxylin and eosin,sequenced,and ***-Seq,Gene Ontology analysis,Kyoto Encyclopedia of Genes and Genomes analysis,and network analyses(Targetscan and miRanda)were used to predict and annotate the circRNA/miRNA/mRNA *** reporter,quantitative reverse transcription polymerase chain reaction,and western blot assays were used to profile expression and regulation patterns of the network in mouse models of ***-eosin staining revealed severe damage to the blood-spinal cord barrier after *** expressed circRNA and miRNA profiles were obtained after TSCI;differentially expressed circRNAs,which were abundant in the cytoplasm,were involved in positive regulation of transcription and protein ***-135b-5p was the most significantly downregulated miRNA after TSCI;circRNAAbca1 and KLF4 were predicted to be its target circRNA and mRNA,***,the circAbca1/miR-135b-5P/KLF4 regulatory axis was predicted and constructed,and its targeted binding was *** inhibiting circAbca1,GAP43 expression was *** expression of circRNAs might play an important role after ***1 plays a neuroinhibitory role by targeted binding of the miR-135b-5P/KLF4 *** identified circRNA/miRNA/mRNA network could provide the basis for understanding pathophysiological mechanisms underlying