Efficient elimination and detection of phenolic compounds in juice using laccase mimicking nanozymes

作     者：Hui Huang Lulu Lei Juan Bai Ling Zhang Donghui Song Jingqi Zhao Jiali Li Yongxin Li Hui Huang;Lulu Lei;Juan Bai;Ling Zhang;Donghui Song;Jingqi Zhao;Jiali Li;Yongxin Li

作者机构：College of Food Science and EngineeringJilin UniversityChangchun 130025China College of New Energy and EnvironmentJilin UniversityChangchun 130021China 

出 版 物：《Chinese Journal of Chemical Engineering》 (中国化学工程学报（英文版）)

年 卷 期：2021年第34卷第1期

页      面：167-175页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 083203[工学-农产品加工及贮藏工程] 

基　　金：financially supported by the National Natural Science Foundation of China (31772058) the Science and Technology Development Project of Jilin Province, China (20190302088GX and 20190701079GH) the Jilin Provincial Strategic Economic Infrastructure Adjustment fund (2019C043-5 and 2020C023-5) Fundamental Research Funds for the Central Universities 

主　　题：Nanomaterials Enzyme Mimic laccase Phenols detection Degradation Juice clarification 

摘      要：Residual phenols in the juice can cause turbidity and affect its sensory *** is used to remove phenolic compounds from fruit juice *** order to overcome the shortcomings of natural laccase instability and high cost,in this work,we prepared a laccase mimic enzyme based on copper ion and adenosine monophosphate(AMP-Cu nanozymes).At the same mass concentration(1 mg·ml^(-1)), the catalytic activity of the nanozyme is about 15 times that of *** AMP-Cu nanozymes had a higher V_(max) and a lower Km than *** laccase mimic enzyme had a good stability under the condition of 30-90 ℃ and pH *** also maintained high catalytic activity at high salt concentrations and 9 days storage ***,the AMP-Cu nanozyme s maintained an initial catalytic activity of about 80% after six consecutive cycles of *** linear range of detection of phenolic compounds by AMP-Cu nanozymes was 0.1-100 μmol·L^(-1) with a detection limit of 0.033 μmol·L^(-1).The phenol removal rate of AMP-Cu nanozymes was much higher than that of laccase under different reaction *** the reaction was performed for 5 h,the phenol removal rate of the fruit juice by AMP-Cu nanozymes was about 65%.The efficient removal of phenolic compounds from different juices by AMP-Cu nanozymes indicate s that they have good application prospect in the food juice industry.