Association of vitamin D receptor gene polymorphism(VDR)with vitamin D deficiency,metabolic and inflammatory markers in Egyptian obese women

作     者：Moushira Zaki Sanaa Kamal Walaa ABasha Eman Youness Wafaa Ezzat Hala El-Bassyouni Khalda Amr 

作者机构：Biological Anthropology DepartmentNational Research CentreGizaEgypt Medical Biochemistry DepartmentNational Research CentreGizaEgypt Internal Medicine DepartmentNational Research CentreGizaEgypt Clinical Genetics DepartmentNational Research CentreCairoEgypt Medical Molecular Genetics DepartmentNational Research CentreEgypt 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2017年第4卷第3期

页      面：176-182页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0703[理学-化学] 10[医学] 

基　　金：This work was supported by grant from National Research Centre Egypt(16361). 

主　　题：Inflammatory biomarkers Obese women Vitamin D deficiency Vitamin D receptor(VDR)gene polymorphism Egyptian 

摘      要：Vitamin D deficiency might contribute to the pathogenesis of metabolic syndrome and could cause immune disturbance.The aim of this study is to analyze the associations between Vitamin D receptor(VDR)gene polymorphism,serum 25-hydroxy vitamin D,metabolic and inflammatory biomarkers in Egyptian obese women.The study included 201 obese women with vitamin D deficiency and 249 obese matched age healthy controls with sufficient vitamin D levels.Their age ranged between 25 and 35 years.Inflammatory biomarkers(interleukin-6 and C-reactive protein)and serum 25(OH)D were measured by enzyme-linked immunosorbent assay.Insulin resistance(IR)was determined by the homeostasis model assessment of insulin resistance(HOMA-IR).Vitamin D receptor(VDR)gene polymorphisms of FokI,ApaI,and TaqI were studied by PCR using the restriction fragment length polymorphism(RFLP)technique.Obese women with vitamin D deficiency had significant higher values of inflammatory and metabolic parameters compared to controls.Multivariable-logistic regression showed associations between 25(OH)D deficiency and metabolic components when comparing cases with controls.Moreover,cases carrying polymorphic alleles showed significant lower levels of serum 25(OH)D and higher HOMA-IR,blood pressure levels and lipid parameters compared to those with the wild type homozygote in obese cases with vitamin D deficiency.Vitamin D deficiency in Egyptian obese women with vitamin D deficiency is associated with abnormal metabolic components and abnormal inflammatory biomarkers.Moreover,VDR polymorphisms play important role in immune and inflammation status.