Protective effect of paeoniflorin on H_(2)O_(2) induced Schwann cells injury based on network pharmacology and experimental validation

作     者：ZHANG Di YANG Bing CHANG Shi-Quan MA Sheng-Suo SUN Jian-Xin YI Lin LI Xing SHI Hui-Mei JING Bei ZHENG Ya-Chun ZHANG Chun-Lan CHEN Feng-Guo ZHAO Guo-Ping ZHANG Di;YANG Bing;CHANG Shi-Quan;MA Sheng-Suo;SUN Jian-Xin;YI Lin;LI Xing;SHI Hui-Mei;JING Bei;ZHENG Ya-Chun;ZHANG Chun-Lan;CHEN Feng-Guo;ZHAO Guo-Ping

作者机构：College of Traditional Chinese MedicineJinan UniversityGuangzhou 510630China LiWan Hospital of Traditional Chinese MedicineGuangzhou 510665China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2021年第19卷第2期

页      面：90-99页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation of China(No.81874404) 

主　　题：Paeoniflorin Hydrogen peroxide H_(2)O_(2) p38MAPK Schwann cells Network pharmacology 

摘      要：This study was to investigate the protective effect of paeoniflorin(PF)on hydrogen peroxide-induced ***,“SMILESof PF was searched in Pubchem and further was used for reverse molecular docking in Swiss Target Prediction database to obtain potential ***-related molecules were obtained from GeenCards database,and the predicted targets of PF for injury treatment were selected by Wayne *** mechanism analysis,the protein-protein interactions were constructed by String,and the KEGG analysis was conducted in ***,cell viability and cytotoxicity assay were established by CCK8 ***,the experimental cells were allocated to control,model(200μmol·L^(−1) H_(2)O_(2)),SB20358010μmol·L^(−1)(200μmol·L^(−1) H_(2)O_(2)+SB20358010μmol·L^(−1)),PF 50μmol·L^(−1)(200μmol·L^(−1) H_(2)O_(2)+PF 50μmol·L^(−1)),and PF 100μmol·L^(−1)(200μmol·L^(−1) H_(2)O_(2)+PF 100μmol·L^(−1))*** measured the intracellular ROS,Hoechst 33258 staining,cell apoptosis,the levels of Bcl-xl,Bcl-2,Caspase-3,Cleaved-caspase3,Cleaved-caspase7,TRPA1,TRPV1,and the phosphorylation expression of *** are 96 potential targets that may be associated with PF for injury ***,we chose the“Inflammatory mediator regulation of TRP channelspathway for the experimental verification from the first 10 KEGG *** experimental verification,H_(2)O_(2) decreased the cell viability moderately(P0.05),and 100μmol·L^(−1) PF increased the cell viability significantly(P0.05).Depending on the difference of intracellular ROS fluorescence intensity,PF inhibited H_(2)O_(2)-induced reactive oxygen species production in Schwann *** Hoechst 33258 staining,PF reversed the condensed chromatin and apoptotic nuclei following H_(2)O_(2) ***,Flow cytometry results showed that PF could substantially inhibit H_(2)O_(2) induced apoptosis(P0.05).Pretreatment with PF obviously reduced the levels of Caspase3,Cleaved-caspase3,Cleaved-caspase7,TRPA1,TRPV1,and the pho