Ethanolic extract of Azadirachta indica ameliorates ovarian defects through phosphoinositide-3 kinase inhibition in a rat model of polycystic ovary syndrome
作者机构：Department of PharmacologyInstitute of PharmacyNirma UniversityAhmadabadGujarat382481India
出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)
年 卷 期：2021年第10卷第1期
页 面：21-28页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：Institute of Pharmacy Nirma University in the form of postgraduate contingency
主 题：Azadirachta indica PI3 kinase Quercetin Steroidogenesis Testosterone propionate Wartmannin
摘 要：Objective:To assess the therapeutic potential of ethanolic extract of Azadirachta(A.)indica in rats with polycystic ovary syndrome(PCOS).Methods:Thirty-five prepubertal female Sprague Dawley rats were randomly divided into five groups with 7 animals in each *** 1 received 0.5%carboxy methyl cellulose *** 2 to 5 received testosterone propionate(0.2 mg/kg,s.c.)dissolved in olive oil daily for 42 days to induce *** addition,group 3 was administered with *** extract(100 mg/kg,0.5%carboxy methyl cellulose orally)from the 7th to 12th week,group 4 received quercetin(100 mg/kg,0.5%carboxy methyl cellulose orally)and group 5 received wartmannin(100 mg/kg,0.5%carboxy methyl cellulose orally).At the end of treatment,blood was collected for biochemical *** follicular count and uterus corpus luteum count followed by PI3K gene expression in the ovary and uterus were ***:The ethanolic extracts of *** significantly reduced body weight,ovary weight and uterus weight of *** of *** also significantly increased the levels of serum glucose,total cholesterol,triglyceride,low-density lipoprotein,very low-density lipoprotein,insulin,testosterone,and luteinizing *** also reduced lipid peroxidation and increased antioxidant parameters in the liver homogenates of PCOS-induced *** examination of the ovary and uterus confirmed PCOS occurrence and remission state in the PCOS-induced and treated groups,***,*** and quercetin significantly downregulated PI3K gene *** results of the ovary and uterus also proved the protective role of ***:*** leaf extract has beneficial effects in the treatment of PCOS by downregulation of PI3K gene expression.
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