Angiogenic peptide hydrogels for treatment of traumatic brain injury

作     者：Xiaotang Ma Agnieszka Agas Zain Siddiqui KaKyung Kim Patricia Iglesias-Montoro Jagathi Kalluru Vivek Kumar James Haorah 

作者机构：Department of Biomedical EngineeringCenter for Injury Bio-MechanicsMaterials and MedicineNew Jersey Institute of TechnologyNewarkNJ07102USA Department of Chemical and Materials EngineeringNew Jersey Institute of TechnologyNewarkNJ07102USA Department of Restorative DentistryRutgers School of Dental MedicineNewarkNJUSA 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2020年第5卷第1期

页      面：124-132页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 

基　　金：This work was supported by grant 1R21AA022734-01A1(to JH)from National Institutes of Health and NIH R15 EY029504 NSF IIP 1903617 the NJIT Undergraduate Research and Innovation(URI)Program and NJIT Startup funds(to V.A.K.). 

主　　题：Traumatic brain injury Cerebrovascular injury Regenerative medicine Angiogenesis Self-assembling peptide hydrogels 

摘      要：Traumatic brain injury(TBI)impacts over 3.17 million Americans.Management of hemorrhage and coagulation caused by vascular disruption after TBI is critical for the recovery of patients.Cerebrovascular pathologies play an important role in the underlying mechanisms of TBI.The objective of this study is to evaluate a novel regenerative medicine for the injured tissue after brain injury.We utilized a recently described synthetic growth factor with angiogenic potential to facilitate vascular growth in situ at the injury site.Previous work has shown how this injectable self-assembling peptide-based hydrogel(SAPH)creates a regenerative microenvironment for neovascularization at the injury site.Supramolecular assembly allows for thixotropy;the injectable drug delivery system provides sustained in vivo efficacy.In this study,a moderate blunt injury model was used to cause physical vascular damage and hemorrhage.The angiogenic SAPH was then applied directly on the injured rat brain.At day 7 post-TBI,significantly more blood vessels were observed than the sham and injury control group,as well as activation of VEGF-receptor 2,demonstrating the robust angiogenic response elicited by the angiogenic SAPH.Vascular markers von-Willebrand factor(vWF)andα-smooth muscle actin(α-SMA)showed a concomitant increase with blood vessel density in response to the angiogenic SAPH.Moreover,blood brain barrier integrity and blood coagulation were also examined as the parameters to indicate wound recovery post TBI.Neuronal rescue examination by NeuN and myelin basic protein staining showed that the angiogenic SAPH may provide and neuroprotective benefit in the long-term recovery.