Toward fine-tuned metabolic networks in industrial microorganisms

作     者：Ning Li Weizhu Zeng Sha Xu Jingwen Zhou 

作者机构：Key Laboratory of Industrial BiotechnologyMinistry of Education and School of BiotechnologyJiangnan University1800 Lihu RoadWuxiJiangsu214122China National Engineering Laboratory for Cereal Fermentation TechnologyJiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Provisional Research Center for Bioactive Product Processing TechnologyJiangnan University1800 Lihu RoadWuxiJiangsu214122China 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2020年第5卷第2期

页      面：81-91页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 0703[理学-化学] 071005[理学-微生物学] 0836[工学-生物工程] 

基　　金：This work was supported by the National Key Research and Development Program of China(2017YFC1600403) the National Science Fund for Excellent Young Scholars(21822806) the National Natural Science Foundation of China(31670095,31770097) the Fundamental Research Funds for the Central Universities(JUSRP51701A) the National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-08). 

主　　题：Fine-tuned regulation Protein engineering Upregulation Downregulation Dynamic regulation 

摘      要：There are numerous microorganisms in nature capable of synthesizing diverse useful compounds;however,these natural microorganisms are generally inefficient in the production of target products on an industrial scale,relative to either chemical synthesis or extraction methods.To achieve industrial production of useful compounds,these natural microorganisms must undergo a certain degree of mutation or effective fine-tuning strategies.This review describes how to achieve an ideal metabolic fine-tuned process,including static control strategies and dynamic control strategies.The static control strategies mainly focus on various matabolic engineering strategies,including protein engineering,upregulation/downregulation,and combinatrorial control of these metabolic engineering strategies,to enhance the flexibility of their application in fine-tuned metabolic metworks.Then,we focus on the dynamic control strategies for fine-tuned metabolic metworks.The design principles derived would guide us to construct microbial cell factories for various useful compounds.