Heterologous Expression of Camellia sinensis Late Embryogenesis Abundant Protein Gene 1(CsLEA1)Confers Cold Stress Tolerance in Escherichia coli and Yeast

作     者：Tong Gao Yunxin Mo Huiyu Huang Jinming Yu Yi Wang Weidong Wang Tong Gao;Yunxin Mo;Huiyu Huang;Jinming Yu;Yi Wang;Weidong Wang

作者机构：College of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2021年第7卷第1期

页      面：89-96页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0807[工学-动力工程及工程热物理] 09[农学] 0901[农学-作物学] 090203[农学-茶学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：This work was supported by the China Postdoctoral Science Foundation(Grant No.2016M602873) the Fundamental Research Funds for the Central Universities(Grant No.2452016182,2452017074) the earmarked fund for Modern Agro-industry Technology Research System(Grant No.CARS-19) the special fund for University-Supported Extension Model(Grant No.TGZX2018-39) 

主　　题：tea plant CsLEA1 heterologous expression cold stress tolerance 

摘      要：Late embryogenesis abundant(LEA)proteins play an important role in plant growth and development,as well as in the plant response to various abiotic *** this study,CsLEA1,a novel gene encoding a LEA_3 subfamily protein,was successfully cloned froma tea plant[Camellia sinensis(L.)***].Bioinformatics analysis and prokaryotic expression assays showed that CsLEA1 is a typical hydrophilic protein with a molecular weight of approximately 10.4 *** analyses revealed that the transcription of CsLEA1 in *** leaves was significantly induced by cold *** addition,the heterologous expression of CsLEA1 increased the tolerance of Escherichia coli and yeast to cold stress,which might be closely related to the low molecular weight and high hydrophilicity of the *** together,our results suggest that CsLEA1 might have an important function in the tolerance of *** to cold stress,thus providing a potential application in molecular breeding to enhance the cold stress tolerance of tea plants.